针对艰难梭菌毒素B的单克隆抗体及其在免疫测定中的应用。

Monoclonal antibodies specific for Clostridium difficile toxin B and their use in immunoassays.

作者信息

Müller F, Stiegler C, Hadding U

机构信息

Institut für Medizinische Mikrobiologie und Virologie, Heinrich-Heine-Universität, Düsseldorf, Germany.

出版信息

J Clin Microbiol. 1992 Jun;30(6):1544-50. doi: 10.1128/jcm.30.6.1544-1550.1992.

DOI:10.1128/jcm.30.6.1544-1550.1992

PMID:1378062

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265326/

Abstract

Five mouse monoclonal antibodies (MAbs) against Clostridium difficile toxin B have been raised and characterized. Three of them were immunoglobulin M (IgM) antibodies (6B10, 6G3, and 10B9), and the other two were of the IgG1 isotype (9E5 and 17G2), recognizing specifically two distinct epitopes on the toxin B molecule. No MAb was able to neutralize cytotoxic activity significantly. The two IgG1 MAbs were purified and applied to various immunodiagnostic assays. MAbs coupled to latex beads were used for specific removal of toxin B from cytotoxic samples and for agglutination assay. An indirect sandwich enzyme-linked immunosorbent assay with MAb 9E5 or 17G2 as the capture antibody was established for identification of toxin B with a lower detection limit of 5 ng/ml.

摘要

已制备并鉴定了五种抗艰难梭菌毒素B的小鼠单克隆抗体（MAb）。其中三种是免疫球蛋白M（IgM）抗体（6B10、6G3和10B9），另外两种是IgG1同种型（9E5和17G2），它们特异性识别毒素B分子上的两个不同表位。没有一种单克隆抗体能够显著中和细胞毒性活性。纯化了两种IgG1单克隆抗体并将其应用于各种免疫诊断试验。与乳胶珠偶联的单克隆抗体用于从细胞毒性样品中特异性去除毒素B以及进行凝集试验。建立了以单克隆抗体9E5或17G2作为捕获抗体的间接夹心酶联免疫吸附测定法，用于鉴定毒素B，检测下限为5 ng/ml。