Chopra M, Beswick H, Clapperton M, Dargie H J, Smith W E, McMurray J
Department of Cardiology, Western Infirmary, Glasgow, Scotland.
J Cardiovasc Pharmacol. 1992 Mar;19(3):330-40. doi: 10.1097/00005344-199203000-00005.
With an assay that generates free radicals (FR) through photooxidation of dianisidine sensitized by riboflavin, 4 x 10(-5) M captopril, epicaptopril (SQ 14,534, captopril's stereoisomer), zofenopril, and fentiapril [all sulfhydryl (-SH)-containing angiotensin-converting enzyme (ACE) inhibitors] were shown effective scavengers of nonsuperoxide free radicals whereas non-SH ACE inhibitors were not. Captopril was a more effective FR scavenger at pH 5.0 than at pH 7.5. Captopril (2 x 10(-5) M) also scavenged the other toxic oxygen species hydrogen peroxide and singlet oxygen and inhibited microsomal lipid peroxidation. Finally, captopril reduced the amount of superoxide anion-radical detected after neutrophils in whole blood were activated with zymosan, probably by inhibiting leukocyte superoxide production.
通过一种利用核黄素敏化的联茴香胺光氧化产生自由基（FR）的检测方法，发现4×10⁻⁵ M的卡托普利、依那普利拉（SQ 14,534，卡托普利的立体异构体）、佐芬普利和芬替普利[所有含巯基（-SH）的血管紧张素转换酶（ACE）抑制剂]是有效的非超氧自由基清除剂，而不含巯基的ACE抑制剂则不是。在pH 5.0时，卡托普利作为FR清除剂比在pH 7.5时更有效。卡托普利（2×10⁻⁵ M）还能清除其他有毒氧物种过氧化氢和单线态氧，并抑制微粒体脂质过氧化。最后，卡托普利可能通过抑制白细胞超氧化物的产生，减少了用酵母聚糖激活全血中的中性粒细胞后检测到的超氧阴离子自由基的量。
