Baier T G, Ludwig W D, Schönberg D, Hartmann K K
Department of Pediatric Endocrinology, University of Heidelberg, Germany.
Eur J Cancer. 1992;28A(6-7):1105-10. doi: 10.1016/0959-8049(92)90466-f.
The expression of insulin-like growth factor I (IGF-I) receptors (IGR-IR) on human B-lineage and T-lineage acute lymphoblastic leukaemias (ALL) representing different maturational stages has been studied. Immature (stage I) and mature (stage II) T ALL as well as pre-B ALL cell lines expressed high numbers of IGF-IR with high affinity for IGF-I. In contrast, on T ALL, stage II and B ALL only low specific binding of 125I-IGF-I was detected. No binding of 125I-IGF-I to Burkitt lymphoma cells was found. Primary human T, pre-B and cALL cells also expressed IGF-IR with Kd for IGF-I and IGF-IR number per cell in the same range as the investigated cell lines. Crosslinking of 125I-IGF-I to T and pre-B ALL cells revealed IGF-IR alpha-subunits of 135 and 116 kD for HSB2. Gene expression of IGF-IR could be detected in all T ALL cell lines but was undetectable in SKW6, a B ALL cell line.
对代表不同成熟阶段的人类B系和T系急性淋巴细胞白血病（ALL）中胰岛素样生长因子I（IGF-I）受体（IGR-IR）的表达进行了研究。不成熟（I期）和成熟（II期）T-ALL以及前B-ALL细胞系表达大量对IGF-I具有高亲和力的IGF-IR。相比之下，在T-ALL II期和B-ALL上仅检测到125I-IGF-I的低特异性结合。未发现125I-IGF-I与伯基特淋巴瘤细胞结合。原代人T、前B和普通ALL细胞也表达IGF-IR，其对IGF-I的解离常数（Kd）和每个细胞的IGF-IR数量与所研究的细胞系处于相同范围。125I-IGF-I与T-ALL和前B-ALL细胞的交联显示，HSB2的IGF-IRα亚基分子量分别为135kD和116kD。在所有T-ALL细胞系中均可检测到IGF-IR的基因表达，但在B-ALL细胞系SKW6中未检测到。
