Tavakkol A, Griffiths C E, Keane K M, Palmer R D, Voorhees J J
Department of Dermatology, University of Michigan Medical Center, Ann Arbor 48109-0528.
J Invest Dermatol. 1992 Aug;99(2):146-50. doi: 10.1111/1523-1747.ep12616781.
We have previously shown that cellular retinoic acid-binding protein II (CRABP-II), but not cellular retinoic acid-binding protein I (CRABP-I), mRNA expression is markedly induced in human skin by topical retinoic acid. In the present study, we have investigated the pattern of expression of CRABP-II transcripts in 4-d RA-treated human skin by non-radioactive in situ hybridization (n = 5) and Northern analysis (n = 4). RA induced accumulation of CRABP-II transcripts throughout the epidermis, dermal fibroblasts, and endothelial cells as determined by in situ hybridization. In skin treated with vehicle, a faint hybridization signal was observed only in basal layers of the epidermis consistent with low-level expression of CRABP-II mRNA. RA-mediated accumulation of CRABP-II transcripts in skin was also confirmed by Northern analysis. Neither RA nor vehicle induced significant changes in nuclear RA receptor-gamma 1 or keratin 5 gene expression in skin as determined by in situ or Northern hybridization. These results indicate that RA-induced CRABP-II mRNA accumulation is primarily localized to spinous and granular layers in epidermis, and in superficial dermis.
我们之前已经表明，局部应用视黄酸可显著诱导人皮肤中细胞视黄酸结合蛋白II（CRABP-II）的mRNA表达，而细胞视黄酸结合蛋白I（CRABP-I）的mRNA表达则未被诱导。在本研究中，我们通过非放射性原位杂交（n = 5）和Northern分析（n = 4）研究了经4天视黄酸处理的人皮肤中CRABP-II转录本的表达模式。原位杂交结果显示，视黄酸诱导CRABP-II转录本在整个表皮、真皮成纤维细胞和内皮细胞中积累。在用赋形剂处理的皮肤中，仅在表皮基底层观察到微弱的杂交信号，这与CRABP-II mRNA的低水平表达一致。Northern分析也证实了视黄酸介导的皮肤中CRABP-II转录本的积累。原位杂交或Northern杂交结果显示，视黄酸和赋形剂均未诱导皮肤中核视黄酸受体γ1或角蛋白5基因表达的显著变化。这些结果表明，视黄酸诱导的CRABP-II mRNA积累主要定位于表皮的棘层和颗粒层以及真皮浅层。
