一种通过磷脂酶C测量胆碱标记的微粒体磷脂酰胆碱水解的简单放射化学分析方法的验证。pH依赖性。
Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence.
作者信息
Cater B R, Trivedi P, Hallinan T
出版信息
Biochem J. 1976 Dec 15;160(3):475-9. doi: 10.1042/bj1600475.
Abstract
Selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by C-type phospholipases from Bacillus cereus or Clostridium perfringens. Both phospholipases substantially hydrolysed phosphatidylcholine over the pH range 4.0-10.0.
摘要
对体内被选择性放射性标记的磷脂酰胆碱种类进行选择性水解，似乎不会干扰蜡样芽孢杆菌或产气荚膜梭菌的C型磷脂酶对微粒体磷脂酰胆碱水解的放射化学测定。在pH值4.0 - 10.0范围内，这两种磷脂酶都能大量水解磷脂酰胆碱。
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2
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J Biol Chem. 1957 May;226(1):497-509.
3
The Ca(2+) stimulated incorporation of choline into microsomal lecithin subspecies in vitro.钙离子在体外刺激胆碱掺入微粒体卵磷脂亚类中。
FEBS Lett. 1971 Sep 15;17(1):14-16. doi: 10.1016/0014-5793(71)80551-3.
4
Phospholipase activity in rat-liver microsomes studied by the use of endogenous substrates.利用内源性底物研究大鼠肝脏微粒体中的磷脂酶活性。
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5
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J Cell Biol. 1969 Feb;40(2):461-83. doi: 10.1083/jcb.40.2.461.
6
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