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1
Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence.一种通过磷脂酶C测量胆碱标记的微粒体磷脂酰胆碱水解的简单放射化学分析方法的验证。pH依赖性。
Biochem J. 1976 Dec 15;160(3):475-9. doi: 10.1042/bj1600475.
2
Enzymatic hydrolysis by bacterial phospolipases C and D of immobilized radioactive sphingomyelin and phosphatidylcholine.细菌磷脂酶C和D对固定化放射性鞘磷脂和磷脂酰胆碱的酶促水解作用。
Acta Pathol Microbiol Scand B. 1981 Oct;89(5):363-7. doi: 10.1111/j.1699-0463.1981.tb00201_89b.x.
3
Inhibition of glucose 6-phosphatase by pure and impure C-type phospholipases. Reactivation by phospholipid dispersions and protection by serum albumin.纯的和不纯的C型磷脂酶对葡萄糖6-磷酸酶的抑制作用。磷脂分散体使其重新激活以及血清白蛋白对其起到保护作用。
Biochem J. 1975 May;148(2):279-94. doi: 10.1042/bj1480279.
4
Phospholipases. I. Effect of n-alkanols on the rate of enzymatic hydrolysis of egg phosphatidylcholine.磷脂酶。I. 正构烷醇对鸡蛋卵磷脂酶促水解速率的影响。
J Membr Biol. 1973 Dec 31;14(2):101-18. doi: 10.1007/BF01868072.
5
Phospholipase C catalyzed formation of sphinogomyelin--14C from lecithin and N-(-14C)-oleoyl-sphingosine.磷脂酶C催化卵磷脂和N-（-14C）-油酰鞘氨醇形成鞘磷脂-14C。
Lipids. 1975 Jul;10(7):391-4.
6
Hydrolytic action of phospholipases on bacterial membranes.磷脂酶对细菌细胞膜的水解作用。
J Biochem. 1977 Nov;82(5):1225-30. doi: 10.1093/oxfordjournals.jbchem.a131809.
7
Asymmetry of the site of choline incorporation into phosphatidylcholine of rat liver microsomes.大鼠肝微粒体中胆碱掺入磷脂酰胆碱位点的不对称性。
Biochim Biophys Acta. 1979 Nov 16;558(1):48-57. doi: 10.1016/0005-2736(79)90314-6.
8
Pathogenicity of Bacillus cereus for insects. I. Production of phospholipase C.蜡样芽孢杆菌对昆虫的致病性。I. 磷脂酶C的产生
Folia Microbiol (Praha). 1972;17(3):221-7. doi: 10.1007/BF02875817.
9
Lecithin biosynthesis in liver mitochondrial fractions.肝脏线粒体组分中的卵磷脂生物合成
Biochem Biophys Res Commun. 1974 May 20;58(2):467-74. doi: 10.1016/0006-291x(74)90388-x.
10
Kinetics of the hydrolysis of monodispersed and micellar phosphatidylcholines catalyzed by a phospholipase C from Bacillus cereus.蜡样芽孢杆菌磷脂酶C催化单分散和胶束磷脂酰胆碱水解的动力学
J Biochem. 1991 Jul;110(1):88-95. doi: 10.1093/oxfordjournals.jbchem.a123547.

引用本文的文献

1
Phospholipid content and activity of pure uridine diphosphate-glucuronyltransferase from rat liver.大鼠肝脏中纯尿苷二磷酸葡萄糖醛酸转移酶的磷脂含量与活性
Biochem J. 1978 Jun 1;171(3):821-4. doi: 10.1042/bj1710821.

本文引用的文献

1
The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins.细菌毒素的生物化学：产气荚膜梭菌毒素的卵磷脂酶活性。
Biochem J. 1941 Sep;35(8-9):884-902. doi: 10.1042/bj0350884.
2
A simple method for the isolation and purification of total lipides from animal tissues.一种从动物组织中分离和纯化总脂质的简单方法。
J Biol Chem. 1957 May;226(1):497-509.
3
The Ca(2+) stimulated incorporation of choline into microsomal lecithin subspecies in vitro.钙离子在体外刺激胆碱掺入微粒体卵磷脂亚类中。
FEBS Lett. 1971 Sep 15;17(1):14-16. doi: 10.1016/0014-5793(71)80551-3.
4
Phospholipase activity in rat-liver microsomes studied by the use of endogenous substrates.利用内源性底物研究大鼠肝脏微粒体中的磷脂酶活性。
Biochim Biophys Acta. 1966 Jun 1;116(3):500-10.
5
Lecithin synthesis, intracellular transport, and secretion in rat liver. IV. A radioautographic and biochemical study of choline-deficient rats injected with choline-3H.大鼠肝脏中卵磷脂的合成、细胞内运输及分泌。IV. 对注射了3H-胆碱的胆碱缺乏大鼠的放射自显影及生化研究。
J Cell Biol. 1969 Feb;40(2):461-83. doi: 10.1083/jcb.40.2.461.
6
The use of radioactive choline as a label for microsomal membranes. II. Studies on membrane-ribosome interaction, subfractionation and function.放射性胆碱作为微粒体膜标记物的应用。II. 膜 - 核糖体相互作用、亚分级分离及功能研究。
Biochim Biophys Acta. 1969 Apr;173(3):554-63. doi: 10.1016/0005-2736(69)90020-0.
7
The use of radioactive choline as a label for microsomal membranes. I. Selectivity of label for endoplasmic reticulum and specificity for lecithin.放射性胆碱作为微粒体膜标记物的应用。I. 对内质网标记的选择性及对卵磷脂的特异性
Biochim Biophys Acta. 1968 Sep 17;163(2):218-25. doi: 10.1016/0005-2736(68)90100-4.
8
Phenobarbital-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum.苯巴比妥诱导的肝内质网中磷脂酰胆碱和甘油三酯合成的改变。
J Lipid Res. 1971 Jan;12(1):1-8.
9
Quantitative studies on the preservation of choline and ethanolamine phosphatides during tissue preparation for electron microscopy. I. Glutaraldehyde, osmium tetroxide, Araldite methods.
J Microsc. 1969;90(1):31-46. doi: 10.1111/j.1365-2818.1969.tb00692.x.
10
The selective loss of lysophospholipids in some commonly used lipid-extraction procedures.某些常用脂质提取方法中溶血磷脂的选择性损失。
Anal Biochem. 1974 Mar;58(1):238-45. doi: 10.1016/0003-2697(74)90463-1.

一种通过磷脂酶C测量胆碱标记的微粒体磷脂酰胆碱水解的简单放射化学分析方法的验证。pH依赖性。

Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence.

作者信息

Cater B R, Trivedi P, Hallinan T

出版信息

Biochem J. 1976 Dec 15;160(3):475-9. doi: 10.1042/bj1600475.

DOI:10.1042/bj1600475
PMID:13785
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164263/
Abstract

Selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by C-type phospholipases from Bacillus cereus or Clostridium perfringens. Both phospholipases substantially hydrolysed phosphatidylcholine over the pH range 4.0-10.0.

摘要

对体内被选择性放射性标记的磷脂酰胆碱种类进行选择性水解，似乎不会干扰蜡样芽孢杆菌或产气荚膜梭菌的C型磷脂酶对微粒体磷脂酰胆碱水解的放射化学测定。在pH值4.0 - 10.0范围内，这两种磷脂酶都能大量水解磷脂酰胆碱。