Naganuma H, Kimura R, Sasaki A, Nukui H, Tasaka K
Department of Neurosurgery, Yamanashi Medical College.
Neurol Med Chir (Tokyo). 1992 Apr;32(4):189-95. doi: 10.2176/nmc.32.189.
This study investigated the secretion of a tumor necrosis factor (TNF) and lymphotoxin (LT) from lymphokine-activated killer (LAK) cells during co-culture with glioblastoma cell lines, autologous glioma cells, and other non-gliomatous tumor cell lines (K562 and Daudi). Cytokine secretion from peripheral blood mononuclear cells (PBMC) was also examined. The TNF activity of culture supernatants was measured by L cell cytotoxic assay, and a neutralization test using anti-TNF and/or anti-LT antibodies determined whether the cytotoxic activity was due to TNF or LT. The results show that LAK cells secrete both TNF and LT during monoculture and release increased amounts of TNF and LT with non-gliomatous tumor cell stimulation, but PBMC secrete only TNF with tumor cell stimulation. Glioblastoma or anaplastic astrocytoma cells, however, did not stimulate cytokine secretion from either LAK cells or PBMC. This indicates a discrepancy between the capability of LAK cells to lyse malignant glioma cells and cytokine secretion from LAK cells, and suggests that malignant glioma cells may produce some factors which inhibit cytokine secretion from LAK cells.
本研究调查了淋巴因子激活的杀伤细胞（LAK细胞）在与胶质母细胞瘤细胞系、自体胶质瘤细胞及其他非胶质瘤肿瘤细胞系（K562和Daudi）共培养期间肿瘤坏死因子（TNF）和淋巴毒素（LT）的分泌情况。还检测了外周血单个核细胞（PBMC）的细胞因子分泌。通过L细胞细胞毒性试验测定培养上清液的TNF活性，并使用抗TNF和/或抗LT抗体进行中和试验，以确定细胞毒性活性是由TNF还是LT引起的。结果显示，LAK细胞在单独培养期间分泌TNF和LT，并且在非胶质瘤肿瘤细胞刺激下释放的TNF和LT量增加，但PBMC在肿瘤细胞刺激下仅分泌TNF。然而，胶质母细胞瘤或间变性星形细胞瘤细胞并未刺激LAK细胞或PBMC分泌细胞因子。这表明LAK细胞裂解恶性胶质瘤细胞的能力与LAK细胞分泌细胞因子之间存在差异，并提示恶性胶质瘤细胞可能产生一些抑制LAK细胞分泌细胞因子的因子。
