Mauzy C A, Hwang O, Egloff A M, Wu L H, Chung F Z
Department of Biotechnology and Pharmacology, Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, Michigan 48105-1047.
Biochem Biophys Res Commun. 1992 Jul 15;186(1):277-84. doi: 10.1016/s0006-291x(05)80804-6.
The human angiotensin II (AII) type 1a receptor gene and its upstream control sequence has been cloned from a human leukocyte genomic library. The promoter element CAAT and TATA sequences were found at -602 and -538, respectively, upstream from the translational initiation site. The deduced protein sequence is homologous to rat and bovine AT1a receptors (94.7% and 95.3% identity). The expressed gene exhibited high-affinity AII and Dup753 binding and was functionally coupled to inositol phosphate turnover. Northern analysis of human tissues showed AT1 receptor mRNA expression in placenta, lung, heart, liver, and kidney. Using 5' untranslated and coding sequence as probes in a Southern blot analysis, it was established that another AT1 subtype exists in the human genome.
人血管紧张素II（AII）1a型受体基因及其上游调控序列已从人白细胞基因组文库中克隆出来。在翻译起始位点上游分别于-602和-538处发现了启动子元件CAAT和TATA序列。推导的蛋白质序列与大鼠和牛的AT1a受体同源（同一性分别为94.7%和95.3%）。表达的基因表现出对AII和Dup753的高亲和力结合，并且在功能上与肌醇磷酸周转偶联。对人体组织的Northern分析表明，AT1受体mRNA在胎盘、肺、心脏、肝脏和肾脏中表达。在Southern印迹分析中使用5'非翻译和编码序列作为探针，确定在人类基因组中存在另一种AT1亚型。
