Matsuzaki Y, Pannetien C, Kanagawa O, Gachelin G, Nakauchi H
Laboratory of Molecular Regulation of Aging, Institute of Physical and Chemical Research (RIKEN), Tsukuba, Japan.
J Immunol. 1992 Aug 1;149(3):1069-74.
MRL mice homozygous for the lpr/lpr gene develop a massive lymphadenopathy caused by the accumulation of CD4-CD8-, Thy-1-positive T cells that express B220. This phenotypically unusual T cell population coexists with normal, B220- T cells in lpr/lpr animals. To investigate the origin and differentiation pathway of B220+ T cells, the expression of a panel of developmentally regulated cell surface markers including TCR, CD4, CD8, Thy-1, and B220 was examined. Thymocytes and peripheral T lymphocytes from lpr/lpr mice were analyzed by four-color flow cytometry. The results showed that both B220+ and B220- thymocytes contained all of CD4-CD8-, CD4+CD8+, and CD4 or CD8 single positive T cell subpopulation in the lpr thymus. Expression of the V beta 11 TCR, measured by flow cytometry and reverse polymerase chain reaction, was demonstrated in lpr thymus. However, the number of T cells expressing V beta 11 was greatly reduced in both the B220+ and B220- T cell populations in lymph node, spleen, and liver. Taken together, the data provide evidence for maturation and selection of a distinct population of B220+ T cells in the thymus of MRL lpr/lpr mice.
纯合lpr/lpr基因的MRL小鼠会因表达B220的CD4-CD8-、Thy-1阳性T细胞的积累而出现严重的淋巴结病。这种表型异常的T细胞群体与lpr/lpr动物体内正常的B220-T细胞共存。为了研究B220+T细胞的起源和分化途径，检测了一组包括TCR、CD4、CD8、Thy-1和B220在内的受发育调控的细胞表面标志物的表达。通过四色流式细胞术分析了lpr/lpr小鼠的胸腺细胞和外周T淋巴细胞。结果显示，lpr胸腺中的B220+和B220-胸腺细胞均包含所有CD4-CD8-、CD4+CD8+以及CD4或CD8单阳性T细胞亚群。通过流式细胞术和逆转录聚合酶链反应检测到lpr胸腺中存在Vβ11 TCR的表达。然而，在淋巴结、脾脏和肝脏的B220+和B220-T细胞群体中，表达Vβ11的T细胞数量均大幅减少。综上所述，这些数据为MRL lpr/lpr小鼠胸腺中一个独特的B220+T细胞群体的成熟和选择提供了证据。
