Chen S L, Hartmann J F
Department of Membrane Biochemistry & Biophysics, Rahway, New Jersey 07065.
Artery. 1992;19(3):177-83.
The influence of growth and aging on the synthesis of DNA, RNA and protein in the myointima in vivo was explored by incubating isolated myointima of different ages in labelled precursors. As myointima grew and aged in vivo, the rates of DNA and protein synthesis declined, while the quantity of DNA increased more than two-fold in seven days. The rate of RNA synthesis remained constant and was unaffected by growth. Like that of DNA and protein synthesis, fetal bovine serum was not required by the isolated myointima to synthesize RNA in vitro. Taken together, these findings confirm and extend our previous results which showed the synthetic autonomy of the isolated myointima, and demonstrate further that during growth the synthesis of RNA is affected in a manner that differs from that which controls DNA and protein.
通过在标记前体中孵育不同年龄的离体肌内膜，探讨了生长和衰老对体内肌内膜中DNA、RNA和蛋白质合成的影响。随着肌内膜在体内生长和衰老，DNA和蛋白质合成速率下降，而DNA含量在7天内增加了两倍多。RNA合成速率保持恒定，不受生长影响。与DNA和蛋白质合成一样，离体肌内膜在体外合成RNA不需要胎牛血清。综上所述，这些发现证实并扩展了我们之前的结果，这些结果表明离体肌内膜具有合成自主性，并进一步证明在生长过程中，RNA的合成受到的影响方式不同于控制DNA和蛋白质合成的方式。
