Sluka K A, Dougherty P M, Sorkin L S, Willis W D, Westlund K N
Marine Biomedical Institute, University of Texas Medical Branch, Galveston 77550-2772.
Brain Res Brain Res Rev. 1992 Jan-Apr;17(1):29-38. doi: 10.1016/0165-0173(92)90004-6.
The effects of an experimentally induced arthritis on immunoreactivity of putative primary afferents neurotransmitter/neuromodulators were examined. Immunoreactive staining for substance P (SP), calcitonin gene-related peptide (CGRP) and glutamate (Glu) in the monkey dorsal horn was examined following inflammation of one knee joint induced by injection of 5% kaolin and 5% carrageenan. Spinal cords were examined at different time periods after induction of arthritis (2.5, 4, 6 and 8 h). Side to side differences in immunoreactivity were determined by a computer assisted quantitation system. A significant overall decrease in immunoreactivity of the lumbar versus the cervical dorsal horn was found for SP. The decrease for SP showed maximal changes of 68.3% at 4 h and 54.7% at 6 h. Immunoreactivity for CGRP was decreased 31.5% at 8 h and variable at other time points. Immunoreactivity for Glu, showed an ipsilateral increase of 31.4% at 4 h, 33.7% at 6 h, 39.9% at 8 h and a significant effect for lumbar versus cervical. Repetitive peripheral stimulation of the joint was shown to be important for changes in SP and Glu immunoreactivity. Without frequent peripheral stimulation in the early stages of the development of arthritis, SP showed no quantitative side to side differences. Increases in Glu immunoreactivity were present but not as prominent with minimal joint manipulation. These studies suggest that Glu may be involved in the aching pain of inflammation at rest whereas SP, CGRP and Glu may mediate pain induced by joint movement.
研究了实验性诱导的关节炎对假定的初级传入神经递质/神经调质免疫反应性的影响。在通过注射5%高岭土和5%角叉菜胶诱导一个膝关节发炎后，检测了猴子背角中P物质（SP）、降钙素基因相关肽（CGRP）和谷氨酸（Glu）的免疫反应性染色。在诱导关节炎后的不同时间段（2.5、4、6和8小时）检查脊髓。通过计算机辅助定量系统确定免疫反应性的左右差异。发现腰椎背角与颈背角相比，SP的免疫反应性总体显著降低。SP的降低在4小时时最大变化为68.3%，在6小时时为54.7%。CGRP的免疫反应性在8小时时降低了31.5%，在其他时间点则有所变化。Glu的免疫反应性在4小时时同侧增加31.4%，在6小时时增加33.7%，在8小时时增加39.9%，并且腰椎与颈椎之间有显著影响。结果表明，关节的重复性外周刺激对于SP和Glu免疫反应性的变化很重要。在关节炎发展的早期阶段，如果没有频繁的外周刺激，SP在数量上没有左右差异。Glu免疫反应性增加，但在最小限度的关节操作下不那么明显。这些研究表明，Glu可能参与静息时炎症的疼痛，而SP、CGRP和Glu可能介导关节运动引起的疼痛。
