Wells V, Mallucci L
Microbiology Department, United Medical School of Guy's Hospital, London, UK.
J Interferon Res. 1992 May;Spec No:51-60. doi: 10.1089/jir.1992.1992.51.
In tertiary MEF undergoing cell cycle progression, autocrine interferon (IFN) is released and constitutive levels of 2',5'-oligoadenylate (2-5A) synthetase activity, low through the cell cycle, surge into a peak within S phase. Treatment of MEF with the autocrine IFN they produce elicits a 2-5A synthetase response from cells positioned in G0 but not from cells in G1 and from cells in S phase. Neutralization of the autocrine IFN by antibody shortens the length of G1 leaving unaltered the kinetics of progression through S and G2 and has no effect on the S phase-linked expression of 2-5A synthetase activity. The growth controlling effect of the autocrine IFN has been mapped to the second part of G1.
在经历细胞周期进程的三级小鼠胚胎成纤维细胞（MEF）中，自分泌干扰素（IFN）被释放，并且2',5'-寡腺苷酸（2-5A）合成酶活性的组成水平在整个细胞周期中较低，但在S期激增到峰值。用它们产生的自分泌IFN处理MEF会引发处于G0期的细胞产生2-5A合成酶反应，但处于G1期的细胞和处于S期的细胞不会产生这种反应。用抗体中和自分泌IFN会缩短G1期的长度，而S期和G2期的进程动力学不变，并且对与S期相关的2-5A合成酶活性表达没有影响。自分泌IFN的生长控制作用已被定位到G1期的第二部分。
