Meijers J H, van der Sanden M P, Tibboel D, van der Kamp A W, Luider T M, Molenaar J C
Department of Paediatric Surgery, Erasmus University, Sophia Children's Hospital, Rotterdam, The Netherlands.
J Pediatr Surg. 1992 Jul;27(7):811-4. doi: 10.1016/0022-3468(92)90371-d.
This study explores the development of the enteric nervous system in avian embryos. Particular emphasis was given to colonization characteristics of neural crest cells present in primitive enteric ganglia. By coculturing neuronal and aneuronal gut of quail and chicken embryos, we investigated if and when neural crest cells in primitive enteric ganglia could detach from these ganglia, migrate, and colonize adjacent chicken gut. Quail neural crest cells were identified using the quail nucleolar marker and the HNK-1 antibody. Enteric neurons were identified using three monoclonal antibodies directed against neurofilament proteins. We found that neural crest cells detached from primitive ganglia in neuronal quail gut from E6 till E9, whereas neural crest cells did not leave enteric ganglia from E10 gut. These observations show that there is a transient phase during which enteric neural crest cells can leave the gut. To determine whether neural crest cells could colonize neuronal gut we cocultured neuronal gut or the neural primordium and neuronal chicken gut (E11). We found that quail neural crest cells do not colonize neuronal E11 gut, whereas they do colonize aneuronal gut of the same age. We suggest that aneuronal gut attracts neural crest cells by diffusing factors.
本研究探讨了鸟类胚胎中肠神经系统的发育。特别强调了原始肠神经节中神经嵴细胞的定植特征。通过将鹌鹑和鸡胚胎的神经元性和非神经元性肠道进行共培养，我们研究了原始肠神经节中的神经嵴细胞是否以及何时能够从这些神经节脱离、迁移并定植于相邻的鸡肠道。使用鹌鹑核仁标记物和HNK - 1抗体来鉴定鹌鹑神经嵴细胞。使用三种针对神经丝蛋白的单克隆抗体来鉴定肠神经元。我们发现，从E6到E9，神经嵴细胞从鹌鹑神经元性肠道的原始神经节脱离，而从E10肠道的神经嵴细胞没有离开肠神经节。这些观察结果表明，存在一个短暂的阶段，在此期间肠神经嵴细胞可以离开肠道。为了确定神经嵴细胞是否能够定植于神经元性肠道，我们将神经元性肠道或神经原基与鸡神经元性肠道（E11）进行共培养。我们发现鹌鹑神经嵴细胞不会定植于E11神经元性肠道，而它们会定植于相同年龄的非神经元性肠道。我们认为非神经元性肠道通过扩散因子吸引神经嵴细胞。
