Lehmann W D, Strecker J R
J Perinat Med. 1976;4(4):255-60. doi: 10.1515/jpme.1976.4.4.255.
In 4 patients with normal pregnancies between the 18th and 20th week of gestation (3H7alpha)-dehydroepiandrosterone-sulfate ([H]-DHEA-S) was injected intraamniotically. Maternal venous blood was drawn before and at regular intervals for 240 minutes after DHEA-injection. Thereafter, legal abortion was performed by intraamniotic instillation of prostaglandine. The conjugated steroids were hydrolyzed enzymatically and the total steroids were isolated and identified. The following labelled metabolites were determined quantitatively: Estriol (e3, estradiol-17beta (E2-17beta), estrone(E1), 16alpha-hydroxy-estrone, (16alpha-OH-DHEA), ALPHA4-androstenedione (AD) and testosterone (T). The maximal increase of all estrogen fractions in matermal plasma occurred 120-180min after intraamniotic injection of the precursor. The most prominent rise of the C18-steroids could be shown for estriol. 60-70% of all metabolites were C16-hydroxylated.
在4例妊娠18至20周的正常孕妇中，羊膜腔内注射了（3H7α）-硫酸脱氢表雄酮（[H]-DHEA-S）。在注射脱氢表雄酮之前及之后的240分钟内定期采集孕妇静脉血。此后，通过羊膜腔内注入前列腺素进行人工流产。对结合类固醇进行酶水解，分离并鉴定总类固醇。定量测定以下标记代谢物：雌三醇（e3）、雌二醇-17β（E2-17β）、雌酮（E1）、16α-羟基雌酮（16α-OH-DHEA）、α4-雄烯二酮（AD）和睾酮（T）。羊膜腔内注射前体后120至180分钟，母体血浆中所有雌激素组分达到最大增幅。雌三醇显示出C18类固醇最显著的升高。所有代谢物的60 - 70%为C16羟基化产物。
