Han Z C, Bikfalvi A, Shen Z X, Bodevin E
Institut des Vaisseaux et du Sang, Hôpital Lariboisière, Paris, France.
Int J Hematol. 1992 Jun;55(3):281-6.
Recombinant acidic human fibroblast growth factor (aFGF) significantly stimulated the formation of megakaryocyte colonies and the size of MK colonies as well as individual MKs in vitro in mice. When aFGF was combined with recombinant mouse interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-6 (IL-6) at their optimal doses, a synergistic action was found between aFGF and IL-3. The activity of aFGF could be completely abrogated by a monoclonal antimouse IL-6 antibody which specifically neutralized the action of mouse IL-6 but not human IL-6. These data indicate that aFGF provides positive growth signals of megakaryocyte progenitor cells, which can give rise to a synergistic action in the presence of IL-3 and which can be abrogated by the antimouse IL-6 antibody.
重组人酸性成纤维细胞生长因子（aFGF）在体外显著刺激了小鼠巨核细胞集落的形成、巨核细胞集落的大小以及单个巨核细胞的生长。当aFGF与重组小鼠白细胞介素-3（IL-3）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）或白细胞介素-6（IL-6）以其最佳剂量联合使用时，发现aFGF与IL-3之间存在协同作用。aFGF的活性可被一种单克隆抗小鼠IL-6抗体完全消除，该抗体可特异性中和小鼠IL-6的作用，但不能中和人IL-6的作用。这些数据表明，aFGF为巨核细胞祖细胞提供了正向生长信号，在IL-3存在时可产生协同作用，且可被抗小鼠IL-6抗体消除。
