Sumida H, Nakamura H, Yasuda M
Department of Anatomy, Hiroshima University School of Medicine, Japan.
Cell Tissue Res. 1992 Apr;268(1):41-9. doi: 10.1007/BF00338052.
Vitronectin is one of the extracellular matrices that mediate cell spreading and attachment in vitro. In the present paper, we demonstrate the involvement of vitronectin in the migration of cushion mesenchymal cells of the embryonic rat heart. Immunohistochemistry established the localization of vitronectin in the myocardial cells and in some of the cushion mesenchymal cells of the truncus arteriosus and atrioventricular canal. In vitro, vitronectin, fibronectin, and collagen type-I revealed significant stimulating activity for cushion mesenchymal cell migration. The distance migrated by cushion mesenchymal cells cultured on vitronectin, collagen type-I, or both vitronectin and fibronectin was similar, but that on fibronectin was significantly shorter. Following the addition of anti-vitronectin IgG to the medium, the migration distance of cushion mesenchymal cells on fibronectin was remarkably increased. Most explants on vitronectin or on both vitronectin and fibronectin became detached from dishes after the addition of the antivitronectin antibody. Immunostaining revealed that cushion mesenchymal cells cultured on substrata other than vitronectin synthesized vitronectin. From these results, it is suggested that vitronectin is synthesized by myocardial cells and some cushion mesenchymal cells, and that vitronectin inhibits cell movement on fibronectin. This feature of vitronectin may be important in the regulation of the migration of cushion mesenchymal cell in vivo.
玻连蛋白是介导细胞在体外铺展和黏附的细胞外基质之一。在本文中，我们证明了玻连蛋白参与胚胎大鼠心脏垫间充质细胞的迁移。免疫组织化学确定了玻连蛋白在心肌细胞以及动脉干和房室管的一些垫间充质细胞中的定位。在体外，玻连蛋白、纤连蛋白和I型胶原对垫间充质细胞迁移显示出显著的刺激活性。在玻连蛋白、I型胶原或玻连蛋白与纤连蛋白两者上培养的垫间充质细胞迁移的距离相似，但在纤连蛋白上的迁移距离明显较短。向培养基中加入抗玻连蛋白IgG后，垫间充质细胞在纤连蛋白上的迁移距离显著增加。加入抗玻连蛋白抗体后，大多数在玻连蛋白或玻连蛋白与纤连蛋白两者上的外植体从培养皿上脱落。免疫染色显示，在除玻连蛋白以外的基质上培养的垫间充质细胞合成玻连蛋白。从这些结果表明，玻连蛋白由心肌细胞和一些垫间充质细胞合成，并且玻连蛋白抑制细胞在纤连蛋白上的移动。玻连蛋白的这一特性可能在体内垫间充质细胞迁移的调节中起重要作用。
