与通用型中和抗体结合的单纯疱疹病毒1型糖蛋白B的表位可引发型特异性抗体依赖性细胞毒性。

Epitopes of herpes simplex virus type 1 glycoprotein B that bind type-common neutralizing antibodies elicit type-specific antibody-dependent cellular cytotoxicity.

作者信息

Sanchez-Pescador L, Paz P, Navarro D, Pereira L, Kohl S

机构信息

Department of Pediatrics, School of Medicine, University of California, San Francisco.

出版信息

J Infect Dis. 1992 Sep;166(3):623-7. doi: 10.1093/infdis/166.3.623.

DOI:10.1093/infdis/166.3.623

PMID:1380051

Abstract

A panel of 45 well-characterized monoclonal antibodies (MAbs) reactive to glycoprotein B (gB) of herpes simplex virus (HSV) type 1 was tested by ELISA and in antiviral functional assays (that included virus neutralization, antibody-dependent cellular cytotoxicity (ADCC), and antibody-dependent complement-mediated lysis), using type 1 or 2 virus strains. All MAbs were ELISA-reactive. Eleven of the MAbs mediated neutralization and 9 mediated ADCC. All of the ADCC epitopes were contained within the amino-terminal half of the extracellular portion of gB. The ADCC reactions were strictly type 1-specific, whereas 9 of 11 neutralizing MAbs exhibited type-common activity. There was some association between the ADCC and neutralization activities, since of 12 MAbs with functional activity, 8 were positive in both assays. These results suggest that differences in the presentation of gB, and perhaps HSV-1 gB versus HSV-2 gB, on free virus and virus-infected cells determine epitope availability.

摘要

利用1型或2型病毒株，通过酶联免疫吸附测定（ELISA）和抗病毒功能测定（包括病毒中和、抗体依赖性细胞毒性（ADCC）以及抗体依赖性补体介导的裂解），对一组45种特性明确的、可与单纯疱疹病毒1型（HSV-1）糖蛋白B（gB）发生反应的单克隆抗体（MAb）进行了检测。所有单克隆抗体在ELISA中均呈反应性。其中11种单克隆抗体介导中和作用，9种介导ADCC。所有ADCC表位均包含在gB胞外部分的氨基末端一半范围内。ADCC反应具有严格的1型特异性，而11种中和单克隆抗体中有9种表现出型共同活性。ADCC和中和活性之间存在一定关联，因为在12种具有功能活性的单克隆抗体中，有8种在两种测定中均呈阳性。这些结果表明，游离病毒和病毒感染细胞上gB的呈现差异，可能还有HSV-1 gB与HSV-2 gB的差异，决定了表位的可及性。