Eizirik D L, Cagliero E, Björklund A, Welsh N
Department of Medical Cell Biology, Uppsala University, Sweden.
FEBS Lett. 1992 Aug 24;308(3):249-52. doi: 10.1016/0014-5793(92)81285-t.
The suppressive and cytotoxic effects of interleukin-1 beta (IL-1 beta) on rodent insulin-producing cells observed in vitro are probably mediated through formation of nitric oxide (NO). In this study we demonstrate that IL-1-induced NO formation in isolated rat islets and insulin-producing HIT cells is more sensitive to inhibition by NG-monomethyl-L-arginine than to inhibition by NG-nitro-L-arginine, thus suggesting that IL-1-exposed insulin-producing cells express an isoform of nitric oxide synthase similar to that present in activated macrophages. Furthermore, IL-1 beta markedly increased the mRNA levels of the inducible macrophage form of nitric oxide synthase in HIT cells.
白细胞介素-1β（IL-1β）在体外对啮齿动物胰岛素生成细胞的抑制和细胞毒性作用可能是通过一氧化氮（NO）的形成介导的。在本研究中，我们证明，IL-1诱导分离的大鼠胰岛和胰岛素生成的HIT细胞中NO的形成对NG-单甲基-L-精氨酸的抑制比对NG-硝基-L-精氨酸的抑制更敏感，因此表明暴露于IL-1的胰岛素生成细胞表达一种与活化巨噬细胞中存在的一氧化氮合酶同工型相似的同工型。此外，IL-1β显著增加了HIT细胞中诱导型巨噬细胞一氧化氮合酶的mRNA水平。
