老年兔视网膜神经节细胞中可溶性蛋白和钙蛋白酶的轴突慢速运输

Slow axonal transport of soluble proteins and calpain in retinal ganglion cells of aged rabbits.

作者信息

Karlsson J O, Inomata M, Kawashima S

机构信息

Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.

出版信息

Neurosci Lett. 1992 Jul 6;141(1):127-9. doi: 10.1016/0304-3940(92)90350-g.

DOI:10.1016/0304-3940(92)90350-g

PMID:1380679

Abstract

The rate of slow axonal transport of soluble proteins in retinal ganglion cells of the rabbit decreased with approximately 25% in aged (6 years) compared to previous estimates in adult (2 years) animals. Immunobinding of calpain to microtiter plates coated with a monoclonal antibody to mu-calpain was used to isolate labelled axonally transported mu-calpain from the nerve extracts. It was found that the distribution of labelled mu-calpain in the retrobulbar optic pathway was similar to the distribution profile of the slowly migrating phase of soluble proteins.

摘要

与成年（2岁）动物之前的估计相比，老年（6岁）兔视网膜神经节细胞中可溶性蛋白质的轴突慢速运输速率下降了约25%。利用钙蛋白酶与包被有抗μ-钙蛋白酶单克隆抗体的微量滴定板的免疫结合，从神经提取物中分离出标记的经轴突运输的μ-钙蛋白酶。研究发现，标记的μ-钙蛋白酶在球后视神经通路中的分布与可溶性蛋白质缓慢迁移阶段的分布情况相似。