Williams L P, Narcessian E J, Andersen O S, Waller G R, Taylor M J, Lazenby J P, Hinton J F, Koeppe R E
Department of Physiology and Biophysics, Cornell University Medical College, New York, New York 10021.
Biochemistry. 1992 Aug 18;31(32):7311-9. doi: 10.1021/bi00147a015.
The gramicidin K family is a set of naturally occurring acylated linear peptides in which a fatty acid is esterified to the ethanolamine hydroxyl of either gramicidin A or C, and possibly also to gramicidin B (Koeppe, R. E., II, Paczkowski, J. A., & Whaley, W. L. (1985) Biochemistry 24, 2822-2826). These acylated gramicidins form membrane-spanning channels in planar lipid bilayers and therefore constitute a model system with which to study the structural and functional consequences of acylation on membrane proteins. This paper serves to characterize further the channels formed by acylated gramicidins A and C and to demonstrate that these channels are structurally equivalent to the channels formed by the standard gramicidins. We also present additional evidence for the ester linkage in the natural acylated gramicidins A and C and identify the fatty acyl chains.
短杆菌肽K家族是一组天然存在的酰化线性肽，其中脂肪酸被酯化到短杆菌肽A或C的乙醇胺羟基上，也可能酯化到短杆菌肽B上（科佩，R.E.二世，帕乔夫斯基，J.A.，& 惠利，W.L.（1985年）《生物化学》24卷，2822 - 2826页）。这些酰化短杆菌肽在平面脂质双分子层中形成跨膜通道，因此构成了一个用于研究酰化对膜蛋白结构和功能影响的模型系统。本文旨在进一步表征酰化短杆菌肽A和C形成的通道，并证明这些通道在结构上等同于标准短杆菌肽形成的通道。我们还提供了关于天然酰化短杆菌肽A和C中酯键的额外证据，并鉴定了脂肪酰链。
