Zhang D, Yeh H H
Department of Neurobiology and Anatomy, University of Rochester Medical Center, NY 14642.
Brain Res Dev Brain Res. 1992 Jul 24;68(1):55-65. doi: 10.1016/0165-3806(92)90247-t.
Substance-P-like immunoreactivity (SP-LI) cells in the Long-Evans rat retina were investigated by combining immunohistochemistry with [3H]thymidine autoradiography. Two subpopulations of SP-LI amacrine cells, with cell bodies in either the proximal portion of the inner nuclear layer (INL) or the ganglion cell layer (GCL), were identified based on morphology, pattern of distribution and development. In the INL, SP-LI cells were found scattered throughout the retina. However, in the GCL, they were limited to the superio-temporal region. Such a contrast in distribution specific to nuclear layers was present upon first detection of SP-LI amacrine cells and persisted throughout development. Birthdating revealed a temporal lag in the histogenesis of SP-LI cells situated in the GCL relative to that in the INL, suggesting that the two subpopulations developed separately. Overall, unique anatomical features of the SP-LI amacrine cells in the rat retina were observed which could only have been uncovered through detailed analyses in the adult as well as during postnatal development.
通过将免疫组织化学与[3H]胸腺嘧啶核苷放射自显影相结合，对Long-Evans大鼠视网膜中P物质样免疫反应性（SP-LI）细胞进行了研究。基于形态、分布模式和发育情况，确定了两类SP-LI无长突细胞亚群，其细胞体分别位于内核层（INL）的近端部分或神经节细胞层（GCL）。在内核层中，SP-LI细胞散在于整个视网膜。然而，在神经节细胞层中，它们仅限于颞上区域。这种核层特异性分布的差异在首次检测到SP-LI无长突细胞时就已存在，并在整个发育过程中持续存在。出生时间测定显示，位于神经节细胞层的SP-LI细胞的组织发生相对于内核层的细胞存在时间滞后，这表明这两个亚群是分开发育的。总体而言，观察到了大鼠视网膜中SP-LI无长突细胞独特的解剖学特征，这些特征只有通过对成年期以及出生后发育过程进行详细分析才能发现。
