Viczian A, Sanyal S, Toffenetti J, Chader G J, Farber D B
Jules Stein Eye Institute, UCLA School of Medicine 90024-7008.
Exp Eye Res. 1992 Jun;54(6):853-60. doi: 10.1016/0014-4835(92)90148-l.
Several retinal mRNAs encoding photoreceptor-specific proteins have been examined in congenic lines of mice carrying different allelic combinations at the rd and rds loci, to determine how mRNA expression is affected by the presence of both the rd and rds genes together or by the presence of one or two rd and rds alleles in the visual cells. Slot blots with retinal RNA from 9 to 30 days old C3H +/+, +/+; rd/+, +/+; rd/rd, +/+; +/+, +/rds; +/+, rds/rds; and the rd/rd, rds/rds double homozygous mutant mice were hybridized successively to several [32P]cDNA probes encoding proteins involved in phototransduction. The increases and decreases in the levels of mRNA coding for opsin, the alpha-subunit of transducin, 48 kDa protein and the beta-subunit of cGMP-phosphodiesterase in the heterozygous and homozygous rds and rd mouse retinas reflected the growth and degeneration of the photoreceptor cells. The heterozygous rd mouse (rd/+, +/+) retina expressed cGMP-phosphodiesterase beta mRNA levels halfway between those in the control (+/+, +/+) and homozygous (rd/rd, +/+) mice, indicating a possible dosage effect. The double homozygous mutant (rd/rd, rds/rds) showed intermediate levels between those observed in the two homozygotes for all mRNAs studied, suggesting a possible interaction between the rd and rds genes.
在携带rd和rds位点不同等位基因组合的近交系小鼠中，研究了几种编码光感受器特异性蛋白的视网膜mRNA，以确定rd和rds基因同时存在或视觉细胞中一个或两个rd和rds等位基因的存在如何影响mRNA表达。用9至30日龄的C3H +/+、+/+；rd/+、+/+；rd/rd、+/+；+/+、+/rds；+/+、rds/rds；以及rd/rd、rds/rds双纯合突变小鼠的视网膜RNA进行狭缝印迹，依次与几种编码参与光转导的蛋白质的[32P]cDNA探针杂交。杂合子和纯合子rds和rd小鼠视网膜中视蛋白、转导蛋白α亚基、48 kDa蛋白和cGMP磷酸二酯酶β亚基编码mRNA水平的增减反映了光感受器细胞的生长和退化。杂合rd小鼠（rd/+、+/+）视网膜中cGMP磷酸二酯酶β mRNA水平介于对照（+/+、+/+）和纯合（rd/rd、+/+）小鼠之间，表明可能存在剂量效应。双纯合突变体（rd/rd、rds/rds）在所有研究的mRNA中显示出介于两个纯合子之间的中间水平，提示rd和rds基因之间可能存在相互作用。
