Jansen I, Uddman R, Ekman R, Olesen J, Ottosson A, Edvinsson L
Department of Experimental Research, Malmö General Hospital, Sweden.
Peptides. 1992 May-Jun;13(3):527-36. doi: 10.1016/0196-9781(92)90084-g.
A sparse to moderate supply of nerve fibers containing neuropeptide Y-like immunoreactivity (NPY-LI), vasoactive intestinal polypeptide (VIP-LI), substance P (SP-LI), and calcitonin gene-related peptide (CGRP-LI) was demonstrated in the walls of human middle meningeal arteries. Comparison with similar studies on human cerebral and temporal arteries indicated a similar distribution and density. The immunoreactive material in all three arterial regions was characterized by reversed-phase high pressure liquid chromatography (HPLC) and radioimmunoassay (RIA). The major peak of NPY-LI, VIP-LI, SP-LI, and CGRP-LI in each extract eluted approximately with the same elution volume as that of the corresponding synthetic analogues. The concentration of NPY in the middle meningeal arteries was lower as compared to the temporal arteries. Low concentrations of SP-LI and CGRP-LI were found in the middle meningeal arteries as compared to the cerebral arteries. In isolated ring segments of human middle meningeal and cerebral arteries, NPY caused vasoconstriction but did not potentiate the contractile response of noradrenaline. In the temporal artery, NPY did not induce contraction but potentiated the vasoconstrictor response to noradrenaline. Vasoactive intestinal polypeptide, peptide histidine methionine-27, SP, neurokinin A, and CGRP relaxed all three types of cephalic arteries. The peptide effects were not antagonized by propranolol, atropine, or cimetidine. Comparison of the responses to VIP and SP of vessels from the different regions showed a similar pattern of reactivity. The response to SP was slightly (p less than 0.05) more potent, whereas the responses to CGRP were less potent in the middle meningeal as compared to that in cerebral (p less than 0.005) vessels.
在人类脑膜中动脉壁中发现了少量至中等数量的含有神经肽Y样免疫反应性（NPY-LI）、血管活性肠多肽（VIP-LI）、P物质（SP-LI）和降钙素基因相关肽（CGRP-LI）的神经纤维。与关于人类大脑动脉和颞动脉的类似研究相比，其分布和密度相似。通过反相高压液相色谱（HPLC）和放射免疫测定（RIA）对所有三个动脉区域中的免疫反应性物质进行了表征。每种提取物中NPY-LI、VIP-LI、SP-LI和CGRP-LI的主要峰与相应合成类似物的洗脱体积大致相同。与颞动脉相比，脑膜中动脉中NPY的浓度较低。与大脑动脉相比，脑膜中动脉中SP-LI和CGRP-LI的浓度较低。在人类脑膜中动脉和大脑动脉的离体环段中，NPY引起血管收缩，但不增强去甲肾上腺素的收缩反应。在颞动脉中，NPY不引起收缩，但增强对去甲肾上腺素的血管收缩反应。血管活性肠多肽、肽组氨酸蛋氨酸-27、SP、神经激肽A和CGRP使所有三种类型的头部动脉舒张。这些肽的作用不受普萘洛尔、阿托品或西咪替丁的拮抗。不同区域血管对VIP和SP反应的比较显示出相似的反应模式。与大脑血管相比，脑膜中动脉对SP的反应稍强（p<0.05），而对CGRP的反应较弱（p<0.005）。
