Mohr F C, Dunston S K
Department of Veterinary Pathology, School of Veterinary Medicine, University of California, Davis 95616.
Am J Vet Res. 1992 May;53(5):820-8.
Mast cells isolated from feline splenic mastocytomas were cultured to study their structural and functional properties. Isolated cells from various cats were grown as monolayer cultures for a mean of 56 days (range, 30 to 76 days). Cat mast cells released allergic mediators in response to compound 48/80, anti-cat serum antibodies, and concanavalin A. On the basis of the finding that secretion from cat mast cells was stimulated by anti-cat serum antibodies and concanavalin A, these cells contain surface-bound immunoglobulins. The presence of mast cell-sensitizing antibodies has been suspected in cats, but never before directly demonstrated. Cultured cat mast cells have cytochemical and functional characteristics common to connective tissue-type mast cells and provide one of the few non-rodent models of cultured cells for the study of this type of mast cell.
从猫脾脏肥大细胞瘤中分离出肥大细胞进行培养，以研究其结构和功能特性。从不同猫身上分离出的细胞作为单层培养物生长，平均培养56天（范围为30至76天）。猫肥大细胞对化合物48/80、抗猫血清抗体和伴刀豆球蛋白A产生反应，释放过敏介质。基于猫肥大细胞的分泌受抗猫血清抗体和伴刀豆球蛋白A刺激这一发现，这些细胞含有表面结合的免疫球蛋白。猫体内一直怀疑存在肥大细胞致敏抗体，但此前从未直接证实过。培养的猫肥大细胞具有结缔组织型肥大细胞共有的细胞化学和功能特征，为研究此类肥大细胞提供了少数非啮齿动物培养细胞模型之一。
