Neskovic N K, Karan V, Budimir M, Vojinovic V, Gasic S, Vitorovic S L
Institute of Pesticides and Environmental Protection, Zemun-Belgrade, Yugoslavia.
J Environ Sci Health B. 1992 Aug;27(4):387-97. doi: 10.1080/03601239209372789.
Wheat grain was treated with 14C-chlorpyrifos-methyl to generate bound residues for determining their bioavailability to rats. In a parallel experiment, bound residues were prepared with non-labelled chlorpyrifos-methyl to determine possible adverse effects in rats fed the grain-bound residue for 28 and 90 days. Two dose levels of 10 and 50 ppm were initially used on the grain. The 10 ppm led to the formation of 25.1% bound residues (2.51 ppm) after 6 months as determined by radiomeasurement. The higher dose was assumed to form 12.55 ppm bound residues. When 14C-bound residues were fed to male rats for 24 hours, the animals eliminated 75% of the radioactivity in urine, 7% in expired air and 8% in faeces after 3 days, indicating that the bound residues were highly bioavailable. A further "bioavailable" amount (4%) was found in selected organs.
用14C-甲基毒死蜱处理小麦籽粒以生成结合残留，用于测定其对大鼠的生物可利用性。在一项平行实验中，用未标记的甲基毒死蜱制备结合残留，以确定给大鼠喂食谷物结合残留28天和90天后可能产生的不良影响。最初在谷物上使用了10 ppm和50 ppm两个剂量水平。通过放射性测量确定，10 ppm剂量在6个月后导致形成25.1%的结合残留（2.51 ppm）。较高剂量假定形成12.55 ppm的结合残留。当将14C结合残留喂给雄性大鼠24小时后，3天后动物在尿液中排出了75%的放射性，在呼出气体中排出7%，在粪便中排出8%，这表明结合残留具有很高的生物可利用性。在选定器官中还发现了另外4%的“生物可利用”量。
