Onoda N, Ohmura E, Tsushima T, Ohba Y, Emoto N, Isozaki O, Sato Y, Shizume K, Demura H
Department of Medicine, Tokyo Women's Medical College, Japan.
Eur J Cancer. 1992;28A(11):1904-9. doi: 10.1016/0959-8049(92)90033-x.
An established cell line (TC-cell, clone 78) derived from human thyroid papillary cancer cells was investigated for production of peptide growth factors. The cells had specific binding sites for insulin-like growth factor-I (IGF-I) and responded to this growth factor with increased proliferation. Culture medium conditioned by TC cells was found to contain insulin-like growth factor (IGF)-I and IGF-binding protein(s). Furthermore, reverse transcription-polymerase chain reaction revealed expression of IGF-I mRNA. When monoclonal antibody to IGF-I receptors (alpha IR3) was added, the growth of TC cells cultured in serum-free medium was significantly reduced. The growth rate of the cells was restored when the antibody was removed from the medium. These results strongly suggest that TC cells produce IGF-I, which is involved in the regulation of their own growth.
研究了一种源自人甲状腺乳头状癌细胞的成熟细胞系（TC细胞，克隆78）产生肽生长因子的情况。这些细胞具有胰岛素样生长因子-I（IGF-I）的特异性结合位点，并对该生长因子产生反应，增殖增加。发现由TC细胞条件培养的培养基含有胰岛素样生长因子（IGF）-I和IGF结合蛋白。此外，逆转录-聚合酶链反应显示IGF-I mRNA的表达。当添加IGF-I受体单克隆抗体（αIR3）时，在无血清培养基中培养的TC细胞的生长显著降低。当从培养基中去除抗体时，细胞的生长速率得以恢复。这些结果强烈表明TC细胞产生IGF-I，其参与自身生长的调节。
