Klein B G, Blaker W D, White C F, Misra B R
Department of Biomedical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Brain Res. 1992 Aug 21;588(2):335-40. doi: 10.1016/0006-8993(92)91596-7.
High-performance liquid chromatography with electrochemical detection (HPLC-ED) and immunocytochemistry were used to examine the time course of serotonergic afferent plasticity within trigeminal subnucleus interpolaris (SpVi) following infraorbital nerve (ION) transection in adult rats. Biochemical analysis was also performed in trigeminal subnucleus caudalis (SpVc) to examine the possibility of transient lesion-induced changes in this region. No significant changes in serotonin (5-HT) or 5-hydroxyindoleacetic acid (5-HIAA) concentration, or in density of 5-HT-immunoreactive (5-HTIR) axonal varicosities were observed in either subnucleus on the lesioned side, up to 51 days following ION cut. However, at 76-79 days post-lesion, a significant increase in 5-HT concentration was again demonstrated within SpVi.
采用高效液相色谱-电化学检测法（HPLC-ED）和免疫细胞化学方法，研究成年大鼠眶下神经（ION）横断后三叉神经中极亚核（SpVi）内血清素能传入可塑性的时间进程。还对三叉神经尾侧亚核（SpVc）进行了生化分析，以研究该区域短暂性损伤诱导变化的可能性。ION切断后长达51天，损伤侧任一亚核内的血清素（5-HT）或5-羟吲哚乙酸（5-HIAA）浓度，或5-HT免疫反应性（5-HTIR）轴突膨体密度均未观察到显著变化。然而，在损伤后76 - 79天，SpVi内5-HT浓度再次显著升高。
