温石棉体外激活人CD4+CD45RA+ T细胞。

Activation of human CD4+CD45RA+ T cells by chrysotile asbestos in vitro.

作者信息

Kinugawa K, Ueki A, Yamaguchi M, Watanabe Y, Kawakami Y, Hyodoh F, Tsushima H

机构信息

Department of Hygiene, Kawasaki Medical School, Kurashiki, Japan.

出版信息

Cancer Lett. 1992 Sep 30;66(2):99-106. doi: 10.1016/0304-3835(92)90221-g.

DOI:10.1016/0304-3835(92)90221-g

PMID:1382835

Abstract

Chrysotile asbestos stimulates T lymphocyte subsets. Cell surface CD4 or CD45RA expression in peripheral blood mononuclear cells (PBMC) was downregulated after incubation with chrysotile asbestos in vitro temporarily. The percentage of CD4+CD45RA+ cells and mean fluorescence intensity in CD4 or CD45RA decreased after incubation with asbestos and returned to the original level after 24 h of incubation, which suggests that chrysotile asbestos activates CD4+CD45RA+ cells. No change was observed in CD29 expression. An increased percentage of IL-2R positive cells and an elevated intracellular Ca++ level were also indicative of the activation of PBMC by chrysotile asbestos.

摘要

温石棉可刺激T淋巴细胞亚群。外周血单个核细胞（PBMC）在体外与温石棉孵育后，细胞表面CD4或CD45RA表达暂时下调。与石棉孵育后，CD4+CD45RA+细胞百分比及CD4或CD45RA中的平均荧光强度降低，孵育24小时后恢复至原始水平，这表明温石棉激活了CD4+CD45RA+细胞。CD29表达未观察到变化。IL-2R阳性细胞百分比增加及细胞内Ca++水平升高也表明温石棉激活了PBMC。