人类同种异体骨髓移植后，CD4⁺和CD8⁺亚群内CD29和CD57标志物频率增加，CD45RA频率降低。

Increased frequencies of the CD29 and CD57 markers and decreased frequency of CD45RA within CD4+ and CD8+ subsets after allogeneic bone marrow transplantation in man.

作者信息

Møller J, Dickmeiss E, Ryder L P, Jacobsen N, Svejgaard A

机构信息

Department of Clinical Immunology, University Hospital (Rigshospitalet), Copenhagen, Denmark.

出版信息

Scand J Immunol. 1991 May;33(5):499-504. doi: 10.1111/j.1365-3083.1991.tb02519.x.

DOI:10.1111/j.1365-3083.1991.tb02519.x

PMID:1709516

Abstract

Two monoclonal antibodies, anti-CD45RA and anti-CD29, reciprocally divide the CD4+ and CD8+ lymphocytes into CD4+ CD45RA+, CD4+ CD29+, CD8+ CD45RA+ and CD8+ CD29+ subsets. The CD4+ CD45RA+, CD4+ CD29+ and CD8+ CD45RA+ possess suppressor-inducer, helper-inducer and suppressor-effector functions respectively. Since the role of these subsets has not been established after allogenic bone marrow transplantation we studied lymphocyte subpopulations in 12 patients 45-227 days after the procedure. The fraction of CD4+ lymphocytes was significantly (P = 0.0005) decreased to 20 +/- 9% versus 43 +/- 3% in controls. Within the CD4+ compartment, we found an increase in the fraction of CD4+ cells that co-expressed CD29 (CD29+/CD4+) to 92 +/- 10% versus 48 +/- 15% (P = 0.008) in controls and a concomitant decrease in CD45RA+/CD4+ to 16 +/- 12% versus 56 +/- 25% (P = 0.008). Patients were also noted to have an increase in the percentage of CD8+ lymphocytes to 41 +/- 5% compared to 23 +/- 4% in controls (P = 0.0004). Examination of the CD8+ subsets revealed a significant increase in the CD29+/CD8+ fraction to 97 +/- 3% versus 64 +/- 2% in controls (P = 0.008) and a decrease in the CD45RA+/CD8+ fraction to 36 +/- 11% versus 70 +/- 21% (P = 0.008). The number of cells co-expressing CD57 were also determined within the CD4+ and CD8+ subsets. In patients CD57+/CD4+ were increased to 29 +/- 7% versus 1 +/- 1% in controls (P = 0.04), and CD57+/CD8+ to 49 +/- 12% versus 23 +/- 9% (P = 0.02). Since CD29+ and CD57+ cells have a poor capability for IL-2 production and proliferation this shift in subset distribution may account for some of the defects in cellular immunity seen within the first year after allogeneic bone marrow transplantation.

摘要

两种单克隆抗体，抗CD45RA和抗CD29，可将CD4⁺和CD8⁺淋巴细胞相互分为CD4⁺CD45RA⁺、CD4⁺CD29⁺、CD8⁺CD45RA⁺和CD8⁺CD29⁺亚群。CD4⁺CD45RA⁺、CD4⁺CD29⁺和CD8⁺CD45RA⁺分别具有抑制诱导、辅助诱导和抑制效应功能。由于这些亚群在异基因骨髓移植后的作用尚未明确，我们对12例患者在移植术后45 - 227天的淋巴细胞亚群进行了研究。CD4⁺淋巴细胞比例显著降低（P = 0.0005），降至20±9%，而对照组为43±3%。在CD4⁺细胞群中，我们发现共表达CD29的CD4⁺细胞比例（CD29⁺/CD4⁺）增加至92±10%，而对照组为48±15%（P = 0.008），同时CD45RA⁺/CD4⁺比例降至16±12%，而对照组为56±25%（P = 0.008）。还发现患者CD8⁺淋巴细胞百分比增加至41±5%，而对照组为23±4%（P = 0.0004）。对CD8⁺亚群的检测显示，CD29⁺/CD8⁺比例显著增加至97±3%，而对照组为64±2%（P = 0.008），CD45RA⁺/CD8⁺比例降至36±11%，而对照组为70±21%（P = 0.008）。还在CD4⁺和CD8⁺亚群中测定了共表达CD57的细胞数量。患者中CD57⁺/CD4⁺增加至29±7%，而对照组为1±1%（P = 0.04），CD57⁺/CD8⁺增加至49±12%，而对照组为23±9%（P = 0.02）。由于CD29⁺和CD57⁺细胞产生白细胞介素 - 2和增殖的能力较差，这种亚群分布的变化可能是异基因骨髓移植后第一年出现的一些细胞免疫缺陷的部分原因。