Segre M, Weigel R M, Schlueter A J, Segre D
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
Cell Immunol. 1992 Oct 15;144(2):324-31. doi: 10.1016/0008-8749(92)90248-n.
The relative affinity and heterogeneity of affinity of idiotypic and anti-idiotypic antibodies in mice immunized with the T-independent antigen DNP-Ficoll and the T-dependent antigen DNP-HGG were measured by a plaque inhibition assay. Idiotypic plaque-forming cells (PFC) were detected by a conventional assay utilizing DNP-coated SRBC. Anti-idiotypic PFC were detected with SRBC coated with affinity-purified anti-DNP antibody of rabbit origin. It was found that both idiotypic and anti-idiotypic antibodies elicited by immunization with the T-independent antigen had lower affinity and were less heterogeneous than the corresponding antibodies originating in mice immunized with the T-dependent antigen. In addition, the affinity and heterogeneity values of the idiotypic antibodies were correlated with the affinity and heterogeneity values of the anti-idiotypic antibodies from the same mice. This finding indicates that idiotypic and anti-idiotypic antibodies mutually regulate each other, thus pointing to internal immunoregulatory effects of the idiotypic network with respect to these parameters.
通过空斑抑制试验测定了用非T细胞依赖性抗原DNP- 聚蔗糖和T细胞依赖性抗原DNP-人γ球蛋白免疫的小鼠中独特型和抗独特型抗体的相对亲和力及亲和力异质性。独特型空斑形成细胞（PFC）通过使用包被有DNP的绵羊红细胞的传统试验来检测。抗独特型PFC则用包被有兔源亲和纯化抗DNP抗体的绵羊红细胞来检测。结果发现，用非T细胞依赖性抗原免疫诱导产生的独特型和抗独特型抗体，与用T细胞依赖性抗原免疫的小鼠产生的相应抗体相比，亲和力较低且异质性较小。此外，同一小鼠的独特型抗体的亲和力和异质性值与抗独特型抗体的亲和力和异质性值相关。这一发现表明独特型和抗独特型抗体相互调节，从而表明独特型网络在这些参数方面具有内在的免疫调节作用。
