Chaye H H, Mauracher C A, Tingle A J, Gillam S
Department of Pathology, University of British Columbia Research Center, Vancouver, Canada.
J Clin Microbiol. 1992 Sep;30(9):2323-9. doi: 10.1128/jcm.30.9.2323-2329.1992.
Better understanding of cell-mediated immune responses to rubella virus would provide the basis for the development of safe and effective vaccines against rubella and would aid in analysis of the pathophysiology of congenital rubella syndrome. We have expressed individual rubella virus structural proteins, E1, E2 and C, via vaccinia virus recombinants. Using the expressed recombinant proteins as antigens, we were able to demonstrate antigen-specific lymphocyte proliferative responses in control individuals and individuals with congenital rubella syndrome. Among the two human groups studied, E1 glycoprotein proved to be a better immunogen than E2 or C. For the control individuals, significant differences in proliferative responses to the structural proteins E1, E2, and C were observed. These differences were not significant in individuals with congenital rubella syndrome. In parallel to the lymphoproliferative responses, immunoglobulin G responses were also found directed mainly to the E1 glycoprotein. These results suggest that E1 may be the most important rubella virus antigen to study in determining the domains required for constructing subunit vaccines against rubella.
更好地理解针对风疹病毒的细胞介导免疫反应将为开发安全有效的风疹疫苗提供基础，并有助于分析先天性风疹综合征的病理生理学。我们通过痘苗病毒重组体表达了风疹病毒的各个结构蛋白，即E1、E2和C。以表达的重组蛋白作为抗原，我们能够在对照个体和先天性风疹综合征患者中证明抗原特异性淋巴细胞增殖反应。在所研究的两组人群中，E1糖蛋白被证明是比E2或C更好的免疫原。对于对照个体，观察到对结构蛋白E1、E2和C的增殖反应存在显著差异。这些差异在先天性风疹综合征患者中不显著。与淋巴细胞增殖反应同时，还发现免疫球蛋白G反应主要针对E1糖蛋白。这些结果表明，在确定构建风疹亚单位疫苗所需的结构域时，E1可能是最重要的风疹病毒抗原。
