结扎导管的大鼠唾液腺中角蛋白、肌动蛋白、S-100蛋白和大鼠表皮生长因子的免疫反应

Immunoreaction of keratin, actin, S-100 protein and rat-EGF in duct-ligated rat salivary glands.

作者信息

Hashimoto J, Yamada K, Ogata K, Takai Y, Mori M

机构信息

Department of Oral and Maxillofacial Surgery, Asahi University School of Dentistry, Gifu, Japan.

出版信息

J Oral Pathol Med. 1992 May;21(5):214-20. doi: 10.1111/j.1600-0714.1992.tb00104.x.

DOI:10.1111/j.1600-0714.1992.tb00104.x

PMID:1383500

Abstract

Duct-ligated submandibular and sublingual glands of rats were evaluated immunohistochemically for changes in keratin (MoAb 1164), actin, S-100 protein and rat-EGF (rEGF). Normal salivary glands were reactive for keratin, S-100 protein and rEGF in the granular convoluted tubule (GCT) and duct cells, and for actin in the myoepithelium. Submandibular glands showed a marked reduction of S-100 protein and rEGF staining following duct ligation, and no increased staining of proliferating epithelial cells of the late stage in duct ligated glands. Sublingual glands revealed no marked changes for actin staining in myoepithelial cells, irrespective of atrophic changes occurring in acinar and duct cells after duct ligation. Immunohistochemical patterns differed for each type of gland; changes associated with the obstructive lesion were more prominent in the submandibular gland.

摘要

对大鼠进行导管结扎的下颌下腺和舌下腺进行免疫组织化学评估，以检测角蛋白（单克隆抗体1164）、肌动蛋白、S-100蛋白和大鼠表皮生长因子（rEGF）的变化。正常唾液腺在颗粒曲管（GCT）和导管细胞中对角蛋白、S-100蛋白和rEGF呈阳性反应，在肌上皮细胞中对肌动蛋白呈阳性反应。导管结扎后，下颌下腺的S-100蛋白和rEGF染色明显减少，导管结扎腺体后期增殖上皮细胞染色未增加。舌下腺肌上皮细胞的肌动蛋白染色无明显变化，无论导管结扎后腺泡和导管细胞是否发生萎缩性变化。每种腺体的免疫组织化学模式不同；与梗阻性病变相关的变化在下颌下腺中更为明显。