Spiegelman V S, Safaev R D, Belitsky G A
Institute of Carcinogenesis, Cancer Research Center, Russian AMS, Moscow.
Mutat Res. 1992 Oct;269(2):185-91. doi: 10.1016/0027-5107(92)90199-c.
Basal levels of aryl hydrocarbon hydroxylase, epoxide hydrolase and glutathione S-transferase enzyme activities, cytochrome P-450 content and inducibility of enzymes with phenobarbital were found to be similar in the microsomes of D. simulans mutant strain 364yv, which is sensitive to the toxic and mutagenic effects of benzo[a]pyrene (BP), and of the wild resistant Turku strain. In contrast, increases in the rate of BP turnover per molecule of cytochrome P-450, intensity of the hemoprotein band with apparent molecular weight 56,000 and the yield of BP 7,8-dihydrodiol and 9,10-dihydrodiol occurred only in microsomes of BP-pretreated 364yv flies but not of Turku ones. It is likely that BP induces an aberrant form of cytochrome P-450 in 364yv flies with a rare mutation in one of the P-450 regulating genes.
在对苯并[a]芘（BP）的毒性和诱变作用敏感的拟暗果蝇突变株364yv以及野生抗性图尔库株的微粒体中，发现芳烃羟化酶、环氧化物水解酶和谷胱甘肽S-转移酶的基础酶活性、细胞色素P-450含量以及苯巴比妥对酶的诱导能力相似。相比之下，每分子细胞色素P-450的BP周转速率增加、表观分子量为56,000的血红蛋白条带强度以及BP 7,8-二氢二醇和9,10-二氢二醇的产量仅在经BP预处理的364yv果蝇的微粒体中出现，而在图尔库果蝇的微粒体中未出现。很可能BP在364yv果蝇中诱导了一种异常形式的细胞色素P-450，其P-450调控基因之一发生了罕见突变。
