甘油和半胱胺对γ射线和重离子辐照后沙门氏菌测试菌株失活和诱变的辐射防护作用。

Radioprotective action of glycerol and cysteamine on inactivation and mutagenesis in Salmonella tester strains after gamma- and heavy ion irradiation.

作者信息

Basha S G, Krasavin E A, Kozubek S

机构信息

Joint Institute for Nuclear Research, Dubna, Moscow Region, Russia.

出版信息

Mutat Res. 1992 Oct;269(2):237-42. doi: 10.1016/0027-5107(92)90205-g.

DOI:10.1016/0027-5107(92)90205-g

PMID:1383707

Abstract

Inactivation and mutagenesis were studied in Salmonella tester strains after gamma-irradiation and after heavy ion irradiation in the presence of glycerol and cysteamine. Ions from deuteron to carbon with residual energies of 2-9 MeV/n were used. Cell sensitivity slightly increased with LET before decreasing. In the presence of glycerol the maximum was shifted to higher values of LET. The radioprotective effect of glycerol for cell killing diminished gradually with increasing LET from 2.0 for gamma-radiation to 1.1 for carbon ions. Mutagenic effectiveness increased slightly for deuterium and helium ions. The protective effect of glycerol decreased with LET but could still be detected for helium ions. The radioprotective effect of cysteamine on mutagenesis was found to be very small in the case of gamma-radiation for the three strains examined.

摘要

研究了在甘油和半胱胺存在的情况下，经γ射线照射和重离子照射后，鼠伤寒沙门氏菌测试菌株中的失活和诱变情况。使用了从氘到碳的离子，剩余能量为2-9 MeV/n。细胞敏感性在降低之前随传能线密度（LET）略有增加。在甘油存在的情况下，最大值向更高的LET值偏移。甘油对细胞杀伤的辐射防护作用随着LET的增加而逐渐减弱，从γ射线的2.0降至碳离子的1.1。氘离子和氦离子的诱变效力略有增加。甘油的保护作用随LET降低，但对氦离子仍可检测到。在所检测的三种菌株中，发现γ射线照射时半胱胺对诱变的辐射防护作用非常小。