Daza P, Escalza P, Mateos S, Cortés F
Departamento de Biología Celular, Facultad de Biologia, Universidad de Sevilla, Spain.
Mutat Res. 1992 Nov 16;270(2):177-83. doi: 10.1016/0027-5107(92)90128-o.
The present study was carried out in order to analyze how persistent the lesions in DNA are which elicit sister-chromatid exchanges (SCEs), induced by three different chemical agents, mitomycin C (MMC), 4-nitroquinoline-1-oxide (4NQO) and ethyl methanesulfonate (EMS), in proliferating human lymphocytes. Cells were exposed to the mutagens for 1 h just before starting bromodeoxyuridine substitution and SCEs were examined in third-cycle metaphases showing three-way-differential staining, by means of our previously standardized method. The results show that, in spite of the fact that these three compounds have different modes of action, the lesions induced by all of them seem to be capable of persisting in DNA and eliciting SCEs for at least three successive cell cycles.
本研究旨在分析由三种不同化学试剂丝裂霉素C（MMC）、4-硝基喹啉-1-氧化物（4NQO）和甲磺酸乙酯（EMS）诱导的、引发姐妹染色单体交换（SCE）的DNA损伤在增殖的人淋巴细胞中持续存在的情况。在开始溴脱氧尿苷替代之前，细胞仅暴露于诱变剂1小时，并通过我们先前标准化的方法，在显示三色差异染色的第三周期中期检查SCE。结果表明，尽管这三种化合物具有不同的作用方式，但它们所诱导的损伤似乎都能够在DNA中持续存在，并在至少三个连续的细胞周期中引发SCE。
