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Large deletions are tolerated at the hprt locus of in vivo derived human T-lymphocytes.

作者信息

Fuscoe J C, Zimmerman L J, Harrington-Brock K, Moore M M

机构信息

Environmental Health Research and Testing, Inc., Research Triangle Park, NC 27709.

出版信息

Mutat Res. 1992 Dec;283(4):255-62. doi: 10.1016/0165-7992(92)90057-o.

DOI:10.1016/0165-7992(92)90057-o
PMID:1383798
Abstract

A cloning assay was used to recover hprt- T-lymphocytes from adult human males. Analysis of crude cellular extracts by polymerase chain reactions (PCRs) demonstrated that 7% (16/218) of the hprt mutations were due to total deletion of the hprt gene. 14 of the 16 mutants were examined by PCR for the presence of flanking DNA to determine the extent of the deletions. The deletion mutation in 13 mutants was at least 350 kb with 5 of these deletions being at least 700 kb. The largest deletions were greater than 15 times the size of the hprt gene. Therefore, large deletions are tolerated at the hprt locus of human T-lymphocytes.

摘要

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