牛胰蛋白酶抑制剂及肾单位细胞中的同源多肽抑制剂

Bovine pancreatic trypsin inhibitor and homologous polypeptide inhibitors in nephron cells.

作者信息

Nori S L, De Renzis G, Fumagalli L, Fioretti E, Barra D, Ascoli F

机构信息

Institute of Anatomy, Catholic University, Rome, Italy.

出版信息

Peptides. 1992 Mar-Apr;13(2):365-71. doi: 10.1016/0196-9781(92)90122-j.

DOI:10.1016/0196-9781(92)90122-j

PMID:1384009

Abstract

Bovine pancreatic trypsin inhibitor (BPTI, aprotinin) is a fifty-eight amino acid polypeptide, which is present together with related molecular isoforms in various bovine organs. In the present study these protease inhibitors were isolated from bovine kidney by affinity chromatography on immobilized trypsin and a subsequent FPLC step. Due to their electrophoretic, structural, and inhibitory properties, the inhibitors were strictly similar to the polypeptides identified previously in other bovine organs. Immunohistochemical experiments showed a widespread localization of these polypeptides in nephron epithelial cells (proximal and distal tubules, loop of Henle, collecting tubules).

摘要

牛胰蛋白酶抑制剂（BPTI，抑肽酶）是一种由58个氨基酸组成的多肽，它与相关的分子异构体一起存在于各种牛器官中。在本研究中，这些蛋白酶抑制剂通过固定化胰蛋白酶上的亲和色谱法以及随后的快速蛋白质液相色谱步骤从牛肾中分离出来。由于它们的电泳、结构和抑制特性，这些抑制剂与先前在其他牛器官中鉴定出的多肽极为相似。免疫组织化学实验表明，这些多肽广泛分布于肾单位上皮细胞（近端和远端小管、亨氏袢、集合小管）中。