表面免疫球蛋白的交联激活了WEHI 231细胞中与src相关的酪氨酸激酶。

Cross-linking of surface immunoglobulin activates src-related tyrosine kinases in WEHI 231 cells.

作者信息

Li Z H, Mahajan S, Prendergast M M, Fargnoli J, Zhu X, Klages S, Adam D, Schieven G L, Blake J, Bolen J B

机构信息

Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.

出版信息

Biochem Biophys Res Commun. 1992 Sep 30;187(3):1536-44. doi: 10.1016/0006-291x(92)90477-3.

DOI:10.1016/0006-291x(92)90477-3

PMID:1384474

Abstract

Crosslinking of sIgM on the B cell line WEHI 231 with anti-sIgM antibody induces protein tyrosine phosphorylation, implicating protein tyrosine kinases (PTKs) in sIg-mediated signal transduction. We have analyzed this cell line for members of the src family of PTKs and have evaluated whether these PTKs might be involved in the process of sIgM-mediated signaling. Our results show that Blk, Lyn, Lck, and Hck are detectable in WEHI 231 cells. Addition of antibodies to sIgM were found to variably stimulate the activities of Blk, Lyn, Lck, and Hck as measured by immune-complex protein kinase assays. Autophosphorylation of these src PTKs, as assessed by reaction with anti-phosphotyrosine antibodies, increased over the time course of sIgM-mediated activation. Co-immunoprecipitation studies to investigate the potential physical interaction of src PTKs with the sIgM receptor complex revealed that, under digitonin and Brij 96 lysis conditions Lyn, Lck, Hck, but not Blk associated with sIgM.

摘要

用抗sIgM抗体交联B细胞系WEHI 231上的sIgM可诱导蛋白酪氨酸磷酸化，这表明蛋白酪氨酸激酶（PTK）参与了sIg介导的信号转导。我们已分析该细胞系中PTK的src家族成员，并评估了这些PTK是否可能参与sIgM介导的信号传导过程。我们的结果显示，在WEHI 231细胞中可检测到Blk、Lyn、Lck和Hck。通过免疫复合物蛋白激酶测定发现，添加抗sIgM抗体可不同程度地刺激Blk、Lyn、Lck和Hck的活性。通过与抗磷酸酪氨酸抗体反应评估，这些src PTK的自身磷酸化在sIgM介导的激活过程中随时间增加。为研究src PTK与sIgM受体复合物潜在的物理相互作用而进行的共免疫沉淀研究表明，在洋地黄皂苷和Brij 96裂解条件下，Lyn、Lck、Hck与sIgM相关联，而Blk不相关。