Kuroda M, Hazama-Shimada Y, Endo A
Biochim Biophys Acta. 1977 Feb 23;486(2):254-9.
Citrinin, a fungal metabolite known as an antibiotic, strongly inhibited the labeled acetate incorporation into nonsaponifiable lipids by a cell-free system from rat liver but not the labeled mevalonate incorporation. Of the enzymes involved in cholesterol synthesis, two enzymes, acetoacetyl-CoA thiolase (EC 2.3.1.9) and 3-hydroxy-3-methylglutaryl-CoA reductase (EC 1.1.1.34), were specifically inhibited by the antibiotic. The concentration required for 50% inhibition was 0.2 mM for the former enzyme and 0.5 mM for the latter. Essentially the same results were obtained with a cell-free system from yeast although higher concentrations of the antibiotic were required for inhibition.
桔霉素是一种作为抗生素的真菌代谢产物，它能强烈抑制大鼠肝脏无细胞体系将标记的乙酸盐掺入非皂化脂质中，但不抑制标记的甲羟戊酸掺入。在参与胆固醇合成的酶中，乙酰乙酰辅酶A硫解酶（EC 2.3.1.9）和3-羟基-3-甲基戊二酰辅酶A还原酶（EC 1.1.1.34）这两种酶受到该抗生素的特异性抑制。前一种酶50%抑制所需浓度为0.2 mM，后一种酶为0.5 mM。用酵母的无细胞体系也得到了基本相同的结果，不过抑制需要更高浓度的抗生素。
