Hirayama C, Sakata M, Ohkura Y, Ihara H, Ohkuma K
Department of Applied Chemistry, Faculty of Engineering, Kumamoto University, Japan.
Chem Pharm Bull (Tokyo). 1992 Aug;40(8):2106-9. doi: 10.1248/cpb.40.2106.
Attempts were made to prepare adsorbents having a high affinity for endotoxin in the culture supernatant of Bordetella pertussis. When poly(gamma-methyl L-glutamate) (PMLG) was used as a matrix and amino groups as the ligand, the highest affinity for endotoxin was attained even at a high ionic strength (mu = 0.2-0.4). PMLG beads containing amino groups of about 3.2 meq/g selectively removed endotoxin from the culture supernatant of B. pertussis without affecting the protective antigens. It was demonstrated that 1 ml of the wet adsorbent adsorbed 4.5 mg of endotoxin. The beads of PMLG derivatives, therefore, are considered to be a useful adsorbent for the removal of endotoxin from the pertussis vaccine, affecting neither filamentous hemagglutinin nor pertussis toxin.
人们尝试制备对百日咳博德特氏菌培养上清液中的内毒素具有高亲和力的吸附剂。当使用聚（γ-甲基-L-谷氨酸）（PMLG）作为基质且氨基作为配体时，即使在高离子强度（μ = 0.2 - 0.4）下也能获得对内毒素的最高亲和力。含有约3.2 meq/g氨基的PMLG珠粒能从百日咳博德特氏菌培养上清液中选择性地去除内毒素，而不影响保护性抗原。结果表明，1 ml湿吸附剂可吸附4.5 mg内毒素。因此，PMLG衍生物珠粒被认为是一种从百日咳疫苗中去除内毒素的有用吸附剂，对丝状血凝素和百日咳毒素均无影响。
