GONZALES F, SOGNNAES R F
Science. 1960 Jan 15;131(3394):156-8. doi: 10.1126/science.131.3394.156.
Electron microscopy of ultrathin osmium-fixed sections of dental calculus, cut with a diamond knife without prior decalcification, revealed densely mineralized areas entrapping many degenerating microorganisms, within which were deposited similarly electron-dense crystals. Two principal forms of crystals were found, the predominant type being of the same order of magnitude and shape as those found in bone, and showing the typical characteristics of apatite in selected area electron diffraction patterns.
对未经预先脱钙、用金刚石刀切割的牙结石超薄锇固定切片进行电子显微镜观察，发现矿化密集区域包裹着许多退化的微生物，其中沉积有同样电子致密的晶体。发现了两种主要的晶体形态，主要类型在大小和形状上与在骨骼中发现的晶体相同，并且在选区电子衍射图中显示出磷灰石的典型特征。
