Hasséssian H, Prat A, Couture R
Department of Physiology, Faculty of Medicine, Université de Montréal, Québec, Canada.
Eur J Pharmacol. 1992 Sep 1;227(1):103-7. doi: 10.1016/0922-4106(92)90150-t.
Basal as well as agonist (100 microM substance P or 2 mM carbachol)-stimulated phosphatidylinositol hydrolysis was investigated in slices of rat spinal cord and cerebral cortex, in the presence and absence of pentobarbital (5-50 microM in vitro or 65 mg/kg in vivo) or urethane (0.2 mM in vitro or 1.4 g/kg in vivo). Urethane was without effect; pentobarbital, however, inhibited the basal hydrolysis and the agonist-stimulated phosphatidylinositol hydrolysis (in dose-dependent fashion in vitro). It is suggested that inhibition of phosphatidylinositol hydrolysis may be part of the cellular mechanisms by which pentobarbital produces anaesthesia.
在存在和不存在戊巴比妥（体外5-50微摩尔或体内65毫克/千克）或乌拉坦（体外0.2毫摩尔或体内1.4克/千克）的情况下，研究了大鼠脊髓和大脑皮层切片中基础以及激动剂（100微摩尔P物质或2毫摩尔卡巴胆碱）刺激的磷脂酰肌醇水解。乌拉坦无作用；然而，戊巴比妥抑制基础水解和激动剂刺激的磷脂酰肌醇水解（在体外呈剂量依赖性）。提示磷脂酰肌醇水解的抑制可能是戊巴比妥产生麻醉作用的细胞机制的一部分。
