Shinozaki H, Ito I, Hasegawa Y, Nakamura K, Igarashi S, Nakamura M, Miyamoto K, Eto Y, Ibuki Y, Minegishi T
Department of Obstetrics and Gynecology, Gunma University School of Medicine, Japan.
FEBS Lett. 1992 Nov 2;312(1):53-6. doi: 10.1016/0014-5793(92)81408-e.
A full-length cDNA for a rat type II activin receptor was cloned by hybridization from a rat ovary cDNA library. The deduced amino acid sequence (513 residues) containing a single membrane-spanning domain and an intracellular kinase domain with predicted serine/threonine specificity. The amino acid sequence is 99.8% and 99.4% identical in the coding region with the previously cloned mouse and human type II activin receptor, and only 66.7% identical in the coding region with the previously cloned rat type IIB activin receptor. We examined the effect of PMSG-hCG on the mRNA level of type II activin receptor in immature rat ovaries. Northern blot analysis of ovarian RNA revealed two mRNAs (3.0 kb and 6.0 kb).
通过从大鼠卵巢cDNA文库杂交克隆出大鼠II型激活素受体的全长cDNA。推导的氨基酸序列（513个残基）包含一个单一的跨膜结构域和一个具有预测的丝氨酸/苏氨酸特异性的细胞内激酶结构域。该氨基酸序列在编码区与先前克隆的小鼠和人类II型激活素受体的同源性分别为99.8%和99.4%，而在编码区与先前克隆的大鼠IIB型激活素受体的同源性仅为66.7%。我们检测了孕马血清促性腺激素-人绒毛膜促性腺激素对未成熟大鼠卵巢中II型激活素受体mRNA水平的影响。卵巢RNA的Northern印迹分析显示有两种mRNA（3.0 kb和6.0 kb）。
