Riegman P H, Vlietstra R J, Suurmeijer L, Cleutjens C B, Trapman J
Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
Genomics. 1992 Sep;14(1):6-11. doi: 10.1016/s0888-7543(05)80275-7.
The human kallikrein gene family is composed of three members: tissue kallikrein (KLK1), prostate-specific antigen (PA or APS), and human glandular kallikrein-1 (hGK-1 or KLK2). The three genes have previously been isolated and mapped to chromosome 19q13.2-q13.4. Further analysis of an area of 110 kb surrounding the kallikrein genes by CHEF electrophoresis and chromosome walking showed clustering of the three genes. The KLK1 gene is positioned in the opposite orientation of the APS and KLK2 genes in the order KLK1-APS-KLK2. The APS and KLK2 gene are separated by 12 kb; the distance between KLK1 and APS is 31 kb. A CpG island was detected in the region between KLK1 and APS. Preliminary data indicate that this CpG island is located directly adjacent to a gene that is unrelated to the kallikreins and seems to be ubiquitously expressed.
组织激肽释放酶（KLK1）、前列腺特异性抗原（PA或APS）和人腺体激肽释放酶-1（hGK-1或KLK2）。这三个基因此前已被分离并定位到19号染色体的13.2-q13.4区域。通过脉冲场凝胶电泳（CHEF）和染色体步移对激肽释放酶基因周围110 kb区域进行的进一步分析显示，这三个基因成簇排列。KLK1基因与APS和KLK2基因的方向相反，排列顺序为KLK1-APS-KLK2。APS和KLK2基因相隔12 kb；KLK1和APS之间的距离为31 kb。在KLK1和APS之间的区域检测到一个CpG岛。初步数据表明，这个CpG岛紧邻一个与激肽释放酶无关的基因，且该基因似乎在全身都有表达。
