Till G O, Lee S, Mulligan M S, Wolter J R, Smith C W, Ward P A, Marak G E
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602.
Invest Ophthalmol Vis Sci. 1992 Nov;33(12):3417-23.
Intraocular accumulation of inflammatory neutrophils is an important feature of experimental phacoanaphylactic endophthalmitis (EPE). Increasing evidence suggests that localization of neutrophils to the site of inflammation requires the participation of neutrophil and endothelial adhesion molecules. These studies were undertaken to determine if blocking of adhesion molecules on neutrophils (CD18) or endothelium (ELAM-1) could attenuate EPE in Lewis rats. Treatment of experimental animals with anti-CD18 or anti-ELAM-1 significantly suppressed intraocular neutrophil accumulation, retinal hemorrhage, and vasculitis, and attenuated retinal edema formation by 48% and 70%, respectively. These observations demonstrate that antibodies directed against adhesion molecules on the neutrophil (CD18) or the vascular endothelial cell (ELAM-1) exhibit potent anti-inflammatory effects, resulting in a striking amelioration of injury in EPE in rats.
炎症性中性粒细胞在眼内积聚是实验性晶状体过敏性眼内炎（EPE）的一个重要特征。越来越多的证据表明，中性粒细胞定位于炎症部位需要中性粒细胞和内皮细胞黏附分子的参与。进行这些研究是为了确定阻断中性粒细胞（CD18）或内皮细胞（ELAM-1）上的黏附分子是否能减轻Lewis大鼠的EPE。用抗CD18或抗ELAM-1治疗实验动物可显著抑制眼内中性粒细胞积聚、视网膜出血和血管炎，并分别使视网膜水肿形成减轻48%和70%。这些观察结果表明，针对中性粒细胞（CD18）或血管内皮细胞（ELAM-1）上黏附分子的抗体具有强大的抗炎作用，可显著改善大鼠EPE中的损伤。
