Van Lookeren Campagne M, Dotti C G, Verkleij A J, Gispen W H, Oestreicher A B
Rudolf Magnus Institute, University of Utrecht, Netherlands.
Neurosci Lett. 1992 Mar 16;137(1):129-32. doi: 10.1016/0304-3940(92)90314-w.
We conducted an electron microscopic immunocytochemical study to locate B-50/GAP43 in various cellular elements of hippocampal neurons grown in dissociated cell culture. B-50 was detected with pre- and post-embedding immunoincubation, using affinity-purified B-50 antibodies and secondary antibodies coated on gold probes. For the first time ultrastructural evidence is presented for the location of B-50 on the membranes of electron-lucent transport vesicles with a diameter of 99.4 +/- 2.9 nm, present in the trans region of the Golgi apparatus, in neurites and in growth cones of cultured hippocampal neurons.
