Mascarello F, Stecchini M L, Rowlerson A, Ballocchi E
Istituto di Anatomia degli Animali Domestici con Istologia ed Embriologia, Facoltà di Medicina Veterinaria, Milano, Italy.
J Anim Sci. 1992 Jun;70(6):1806-13. doi: 10.2527/1992.7061806x.
The postnatal development of skeletal muscles was studied in growing pigs from 8 to 210 d of age. Indirect immunoperoxidase staining of frozen sections of porcine semimembranosus muscle and longissimus muscle revealed a distinct population of small fibers (tertiary myotubes) that were stained specifically by an antibody (anti-NE) selective for the developmental (embryonic and neonatal) isoforms of muscle myosin. At 8 d of age the other larger fibers were already anti-NE negative and differentiated into Types I and II. A gradual decrease in the number of anti-NE positive fibers together with a gradual increase in area of the remaining positive fibers was observed throughout the pigs' growth. These results may indicate that hyperplastic growth does not cease at birth. Possible mechanisms to explain the origin of these tertiary myotubes containing developmental isoforms of myosin are suggested.
对8至210日龄生长猪的骨骼肌产后发育进行了研究。对猪半膜肌和背最长肌冰冻切片进行间接免疫过氧化物酶染色，发现有一群独特的小纤维（三级肌管），它们被一种对肌肉肌球蛋白的发育（胚胎和新生）异构体具有选择性的抗体（抗NE）特异性染色。在8日龄时，其他较大的纤维已经抗NE阴性，并分化为I型和II型。在猪的整个生长过程中，观察到抗NE阳性纤维数量逐渐减少，同时剩余阳性纤维的面积逐渐增加。这些结果可能表明，增生性生长在出生时并未停止。文中提出了解释这些含有肌球蛋白发育异构体的三级肌管起源的可能机制。
