心房利钠肽和脑利钠肽可增强培养的大鼠下丘脑细胞（包括多巴胺能神经元）中的多巴胺积累。

Atrial and brain natriuretic peptides enhance dopamine accumulation in cultured rat hypothalamic cells including dopaminergic neurons.

作者信息

Kadowaki K, Hirota K, Koike K, Ohmichi M, Miyake A, Tanizawa O

机构信息

Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.

出版信息

Neuroendocrinology. 1992 Jul;56(1):11-7. doi: 10.1159/000126202.

DOI:10.1159/000126202

PMID:1386413

Abstract

Dopamine accumulation in hypothalamic cells by atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) was analyzed using newborn rat hypothalamic cells in culture. Both ANP and BNP caused a dose-dependent increase in [3H]-dopamine accumulation in the cells. ANP increased [3H]-dopamine accumulation significantly within 20 min. The effects of ANP and BNP on dopamine accumulation paralleled an increase in intracellular cGMP concentration. (Bu)2-cGMP and sodium nitroprusside, a stimulator of the soluble form of guanylate cyclase, also enhanced [3H]-dopamine accumulation. ANP had no effect on efflux of [3H] radioactivity after [3H]-dopamine uptake. These results suggest that a change in cGMP is one of the intermediate steps in dopamine accumulation in hypothalamic cells by ANP and BNP.

摘要

利用新生大鼠下丘脑培养细胞，分析了心房利钠肽（ANP）和脑利钠肽（BNP）在下丘脑细胞中对多巴胺蓄积的影响。ANP和BNP均使细胞内[3H]-多巴胺蓄积呈剂量依赖性增加。ANP在20分钟内显著增加了[3H]-多巴胺的蓄积。ANP和BNP对多巴胺蓄积的作用与细胞内cGMP浓度的增加平行。(Bu)2-cGMP和可溶性鸟苷酸环化酶刺激剂硝普钠也增强了[3H]-多巴胺的蓄积。ANP对[3H]-多巴胺摄取后[3H]放射性流出没有影响。这些结果表明，cGMP的变化是ANP和BNP在下丘脑细胞中导致多巴胺蓄积的中间步骤之一。