测定血清铁和铁结合能力的快速增量法。
Rapid incremental methods for the determination of serum iron and iron-binding capacity.
作者信息
BEALE R N, BOSTROM J O, TAYLOR R F
出版信息
J Clin Pathol. 1961 Sep;14(5):488-95. doi: 10.1136/jcp.14.5.488.
Abstract
Rapid methods depending on differential absorptiometry are described for the determination of the transferrin iron content and the latent iron-binding capacity of blood serum. Each determination requires as little as 0.5 ml. serum. The methods are well adapted for routine use in the ;average' laboratory. Three or four sera may be completely analysed in 30 minutes. All operations are carried out in the cells or tubes used for the colorimetric measurements, no precipitation or heating being employed at any stage. Critical investigations of the reliability of the methods are attempted and ranges of normal values are included.
摘要
本文描述了基于差示吸光测定法的快速方法，用于测定血清中转铁蛋白铁含量和潜在铁结合能力。每次测定仅需0.5毫升血清。这些方法非常适合普通实验室的常规使用。30分钟内可对三到四份血清进行完整分析。所有操作均在用于比色测量的细胞或试管中进行，任何阶段均不采用沉淀或加热操作。本文尝试对这些方法的可靠性进行关键研究，并给出了正常数值范围。
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