对粗糙脉孢菌野生型和迟缓型菌株线粒体通透性的测量。
Permeability measurements on mitochondria from wild-type and poky strains of Neurospora crassa.
作者信息
Katkocin D M, Slayman C W
出版信息
J Bacteriol. 1976 Sep;127(3):1270-7. doi: 10.1128/jb.127.3.1270-1277.1976.
The permeability properties of isolated Neurospora mitochondria were determined by measuring the rate at which the mitochondria swell in isotonic solutions of various organic and inorganic molecules. Like mammalian mitochondria, wild-type Neurospora mitochondria were impermeable to sucrose and only slightly more permeable to most inorganic ions (K, Na, Cl). Their permeability to K was greatly increased by valinomycin and by monensin. In addition, the mitochondria contain specific systems mediating PO4 uptake and PO4- malate, fumarate, and succinate exchange. Mitochondria from the maternally inherited poky strain of Neurospora, previously demonstrated to possess defective ribosomes and a grossly cytochrome chain, showed a slight but significant increase in permeability to inorganic ions. They contained, however, the specific uptake and exchange systems for phosphate and dicarboxylate anions, a result suggesting that these systems do not depend upon mitochondrially synthesized polypeptides.
通过测量线粒体在各种有机和无机分子等渗溶液中的肿胀速率，来确定分离出的粗糙脉孢菌线粒体的通透性特性。与哺乳动物线粒体一样，野生型粗糙脉孢菌线粒体对蔗糖是不透性的，而对大多数无机离子（钾、钠、氯）的通透性仅略高一点。缬氨霉素和莫能菌素可大大增加其对钾的通透性。此外，线粒体含有介导磷酸根摄取以及磷酸根 - 苹果酸、富马酸和琥珀酸交换的特定系统。先前已证明，来自母系遗传的粗糙脉孢菌迟缓菌株的线粒体拥有缺陷核糖体和严重受损的细胞色素链，其对无机离子的通透性略有但显著增加。然而，它们含有磷酸盐和二羧酸阴离子的特定摄取和交换系统，这一结果表明这些系统不依赖于线粒体合成的多肽。
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