BROBERGER O, PERLMANN P
J Exp Med. 1962 Jan 1;115(1):13-26. doi: 10.1084/jem.115.1.13.
Thirteen sera from children with ulcerative colitis were examined for antibodies reacting with constituents of human colonic tissue by means of immunofluorescent methods. 3 out of 10 sera reacted positively when tested by the direct staining method while 6 out of 13 reacted positively when tested by the indirect method with conjugates of rabbit anti-human gamma globulin. The specificity of the reactions could be confirmed by inhibition tests. 16 sera from healthy children and adults yielded completely negative results. The staining capacity of various sera was correlated to their hemagglutinating titer when they were tested with sheep erythrocytes, coated with phenol-water extract of human colon. Absorption experiments indicated that the stainable antigen was also present in the extracts used for the hemagglutination experiments. In unfixed tissue sections, fluorescent antibodies were adsorbed onto the epithelial cells of the mucosa. Adsorption on epithelial basement membranes could not be demonstrated. Fluorescent H agglutinins, isolated from eel serum, were adsorbed onto the same mucosal structures of human colon (blood group O) as the antibodies in the sera of patients with ulcerative colitis. However, any immunological relationship between H substance and the colonic antigen of ulcerative colitis could be ruled out by cross-inhibition and hemagglutination inhibition experiments. Fluorescent serum from patients with rheumatoid arthritis also stained sections of human colon but the localization of the stainable antigens was different from that visualized with the ulcerative colitis sera. Inhibition experiments indicated that the rheumatoid arthritis serum contained antibodies staining colon antigens different from those reacting with antibodies in the ulcerative colitis sera. Sera from patients with systemic lupus erythematosus or with the nephrotic syndrome, which all hemagglutinated erythrocytes coated with colon extract, did not stain the sections of the colon tissue either because of suboptimal antibody concentration or because of a difference in type or localization of the antigen.
采用免疫荧光法检测了13份溃疡性结肠炎患儿血清中与人类结肠组织成分发生反应的抗体。直接染色法检测时,10份血清中有3份呈阳性反应;用兔抗人丙种球蛋白结合物进行间接法检测时,13份血清中有6份呈阳性反应。反应的特异性可通过抑制试验来证实。16份健康儿童和成人的血清结果均为完全阴性。用涂有人结肠酚水提取物的绵羊红细胞检测各种血清时,其染色能力与其血凝效价相关。吸收实验表明,可染色抗原也存在于用于血凝实验的提取物中。在未固定的组织切片中,荧光抗体吸附在黏膜上皮细胞上。未证实其吸附在上皮基底膜上。从鳗鱼血清中分离出的荧光H凝集素与溃疡性结肠炎患者血清中的抗体一样,吸附在人类结肠(O血型)的相同黏膜结构上。然而,通过交叉抑制和血凝抑制实验可排除H物质与溃疡性结肠炎结肠抗原之间的任何免疫关系。类风湿性关节炎患者的荧光血清也能使人类结肠切片染色,但可染色抗原的定位与溃疡性结肠炎血清所显示的不同。抑制实验表明,类风湿性关节炎血清中所含抗体对结肠抗原的染色与溃疡性结肠炎血清中抗体所反应的抗原不同。系统性红斑狼疮或肾病综合征患者的血清,虽然都能使涂有结肠提取物的红细胞发生血凝,但由于抗体浓度欠佳或抗原类型或定位不同,均未使结肠组织切片染色。